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  Evaluation of the LightCycler(R) 1536 Instrument for high-throughput quantitative real-time PCR

Schlesinger, J., Tönjes, M., Schueler, M., Zhang, Q., Dunkel, I., & Sperling, S. R. (2010). Evaluation of the LightCycler(R) 1536 Instrument for high-throughput quantitative real-time PCR. Methods, 50(4), S19-S22. doi:10.1016/j.ymeth.2010.01.007.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7BA5-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7BA6-2
Genre: Journal Article
Alternative Title : Methods

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 Creators:
Schlesinger, J.1, Author              
Tönjes, M.1, Author              
Schueler, M.1, Author              
Zhang, Q.1, Author              
Dunkel, I.2, Author              
Sperling, S. R.3, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Computational Epigenetics (Ho-Ryun Chung), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479658              
3Max Planck Society, ou_persistent13              

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Free keywords: LightCycler® 1536; Hydrolysis probe assays; Gene expression; Real-time PCR; Quantitative real-time PCR
 Abstract: Quantitative real-time PCR (qPCR) is a frequently used, sensitive and accurate method to study gene expression profiles. However, its throughput was so far limited for routine laboratories to 384 reactions per run based on the limitations of the available instruments. Recently, the LightCycler(R) 1536 Instrument was launched providing a high-throughput solution for qPCR with the analysis of 1536 reactions in approximately 45min. We assessed the accuracy and sensitivity of this novel technology for the analysis of gene expression profiles in combination with the Innovadyne Nanodrop Express pipetting robot. We compared expression profiles obtained for 42 genes in 71 samples between the Universal ProbeLibrary and the LightCycler(R) 1536 Instrument and SYBR Green I and the ABI PRISM 7900HT system. We found that the results were highly reproducible between both systems. Beside the higher throughput, the advantage of the LightCycler(R) 1536 Instrument was the reduced consumption of reagents and sample material.

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 Dates: 2010-03-07
 Publication Status: Published in print
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Title: Methods
  Alternative Title : Methods
Source Genre: Journal
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Pages: - Volume / Issue: 50 (4) Sequence Number: - Start / End Page: S19 - S22 Identifier: ISSN: 1046-2023