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  A calibrated diversity assay for nucleic acid libraries using DiStRO-a Diversity Standard of Random Oligonucleotides

Schütze, T., Arndt, P. F., Menger, M., Wochner, A., Vingron, M., Erdmann, V. A., et al. (2010). A calibrated diversity assay for nucleic acid libraries using DiStRO-a Diversity Standard of Random Oligonucleotides. Nucleic Acids Research, 38(4), e23-e23. doi:doi:10.1093/nar/gkp1108.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7BC8-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7BC9-3
Genre: Journal Article
Alternative Title : Nucleic Acids Res

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 Creators:
Schütze, T.1, Author              
Arndt, P. F.2, Author              
Menger, M., Author
Wochner, A., Author
Vingron, M.3, Author              
Erdmann, V. A.4, Author
Lehrach, H.1, Author              
Kaps, C., Author
Glökler, J.1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Evolutionary Genomics (Peter Arndt), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479638              
3Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              
4Max Planck Society, ou_persistent13              

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Free keywords: Calibration; DNA/standards; *Gene Library; Kinetics; Nucleic Acid Denaturation; Oligodeoxyribonucleotides/chemical synthesis/*standards; Reference Standards; SELEX Aptamer Technique; Temperature
 Abstract: We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as the best indicator of complexity. Direct comparisons of nucleic acid pools obtained from an aptamer selection demonstrate that even highly complex populations can be evaluated by using DiStRO, without the need of complicated calculations.

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Language(s): eng - English
 Dates: 2010-03
 Publication Status: Published in print
 Pages: -
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Title: Nucleic Acids Research
  Alternative Title : Nucleic Acids Res
Source Genre: Journal
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Pages: - Volume / Issue: 38 (4) Sequence Number: - Start / End Page: e23 - e23 Identifier: ISSN: 1362-4962 (Electronic) 0305-1048 (Linking) %R gkp1108 [pii] 10.1093/nar/gkp1108