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  alpha-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel

Bhushan, S., Gartmann, M., Halic, M., Armache, J. P., Jarasch, A., Mielke, T., et al. (2010). alpha-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel. Nature Structural & Molecular Biology, 17(3), 313-317. doi:10.1038/nsmb.1756.

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Genre: Journal Article
Alternative Title : Nat Struct Mol Biol

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 Creators:
Bhushan, S., Author
Gartmann, M., Author
Halic, M., Author
Armache, J. P., Author
Jarasch, A., Author
Mielke, T.1, Author              
Berninghausen, O., Author
Wilson, D. N.2, Author              
Beckmann, R., Author
Affiliations:
1Imaging/Electron Microscopy (Head: Rudi Lurz/Thorsten Mielke), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Computer Simulation; Cryoelectron Microscopy; Image Processing, Computer-Assisted; Models, Biological; Peptides/chemistry/metabolism; Protein Biosynthesis; Protein Conformation; Protein Folding; Ribosomes/chemistry/metabolism/ultrastructure
 Abstract: As translation proceeds, the nascent polypeptide chain passes through a tunnel in the large ribosomal subunit. Although this ribosomal exit tunnel was once thought only to be a passive conduit for the growing nascent chain, accumulating evidence suggests that it may in fact play a more active role in regulating translation and initial protein folding events. Here we have determined single-particle cryo-electron microscopy reconstructions of eukaryotic 80S ribosomes containing nascent chains with high alpha-helical propensity located within the exit tunnel. The maps enable direct visualization of density for helices as well as allowing the sites of interaction with the tunnel wall components to be elucidated. In particular regions of the tunnel, the nascent chain adopts distinct conformations and establishes specific contacts with tunnel components, both ribosomal RNA and proteins, that have been previously implicated in nascent chain-ribosome interaction.

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Language(s): eng - English
 Dates: 2010-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 542341
DOI: 10.1038/nsmb.1756
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Title: Nature Structural & Molecular Biology
  Alternative Title : Nat Struct Mol Biol
Source Genre: Journal
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Pages: - Volume / Issue: 17 (3) Sequence Number: - Start / End Page: 313 - 317 Identifier: ISSN: 1545-9985 (Electronic)