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  Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group

Meyerdierks, A., Kube, M., Kostadinov, I., Teeling, H., Glöckner, F. O., Reinhardt, R., et al. (2010). Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group. Environmental Microbiology, 12(2), 422-439. doi:10.1111/j.1462-2920.2009.02083.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7BFE-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7BFF-D
Genre: Journal Article
Alternative Title : Environ Microbiol

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 Creators:
Meyerdierks, Anke1, Author
Kube, Michael2, Author              
Kostadinov, Ivaylo1, Author
Teeling, Hanno2, Author              
Glöckner, Frank Oliver1, Author
Reinhardt, Richard2, Author              
Amann, Rudolf, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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 Abstract: Microbial consortia mediating the anaerobic oxidation of methane with sulfate are composed of methanotrophic Archaea (ANME) and Bacteria related to sulfate-reducing Deltaproteobacteria. Cultured representatives are not available for any of the three ANME clades. Therefore, a metagenomic approach was applied to assess the genetic potential of ANME-1 archaea. In total, 3.4 Mbp sequence information was generated based on metagenomic fosmid libraries constructed directly from a methanotrophic microbial mat in the Black Sea. These sequence data represent, in 30 contigs, about 82–90% of a composite ANME-1 genome. The dataset supports the hypothesis of a reversal of the methanogenesis pathway. Indications for an assimilatory, but not for a dissimilatory sulfate reduction pathway in ANME-1, were found. Draft genome and expression analyses are consistent with acetate and formate as putative electron shuttles. Moreover, the dataset points towards downstream electron-accepting redox components different from the ones known from methanogenic archaea. Whereas catalytic subunits of [NiFe]-hydrogenases are lacking in the dataset, genes for an [FeFe]-hydrogenase homologue were identified, not yet described to be present in methanogenic archaea. Clustered genes annotated as secreted multiheme c-type cytochromes were identified, which have not yet been correlated with methanogenesis-related steps. The genes were shown to be expressed, suggesting direct electron transfer as an additional possible mode to shuttle electrons from ANME-1 to the bacterial sulfate-reducing partner.

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Language(s): eng - English
 Dates: 2010-02
 Publication Status: Published in print
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Title: Environmental Microbiology
  Alternative Title : Environ Microbiol
Source Genre: Journal
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Pages: - Volume / Issue: 12 (2) Sequence Number: - Start / End Page: 422 - 439 Identifier: ISSN: 1462-2912