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  FISH as a tool to investigate chromosome behaviour in budding yeast.

Scherthan, H., & Loidl, J. (2010). FISH as a tool to investigate chromosome behaviour in budding yeast. In J. M. Bridger, & E. V. Volpi (Eds.), Fluorescence in situ Hybridization (FISH): Protocols and Applications (pp. 363-377). New York [et al]: Springer.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7C2F-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7C30-4
Genre: Book Chapter

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 Creators:
Scherthan, Harry1, Author              
Loidl, Josef, Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: DNA labeling; FISH; GISH; Immunofluorescence; Saccharomyces cerevisiae; Chromosome painting; Chromosome dynamics
 Abstract: Fluorescence in situ hybridization (FISH) provides an effective means to delineate chromosomes and their subregions during all stages of the cell cycle. This makes FISH particularly useful for studying chromosome behavior in species with minute genomes and/or poor chromosome condensation at metaphase, which is the case in model organisms such as the budding yeast Saccharomyces cerevisiae. Since its introduction in 1992, FISH with composite whole chromosome or locus specific probes has become an indispensable tool in the analysis of chromosome behavior in metaphase and interphase cells, and especially of meiotic chromosome pairing of wild-type and mutant yeast strains.

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Language(s): eng - English
 Dates: 2010-01-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

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Title: Fluorescence in situ Hybridization (FISH) : Protocols and Applications
Source Genre: Book
 Creator(s):
Bridger, Joanna M., Editor
Volpi, Emanuela V., Editor
Affiliations:
-
Publ. Info: New York [et al] : Springer
Pages: xiv, 451 Volume / Issue: - Sequence Number: - Start / End Page: 363 - 377 Identifier: -

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Title: Methods in molecular biology
Source Genre: Series
 Creator(s):
Walker, John M., Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: 659, Part 4, Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745