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  Dicer is required for Sertoli cell function and survival

Kim, G.-J., Georg, I., Scherthan, H., Merkenschlager, M., Guillou, F., Scherer, G., et al. (2010). Dicer is required for Sertoli cell function and survival. International Journal of Developmental Biology, 54, 867-875. doi:10.1387/ijdb.092874gk.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7C4E-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7C4F-F
Genre: Journal Article
Alternative Title : Int J Dev Biol

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Kim.pdf (Any fulltext), 780KB
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 Creators:
Kim, Gwang-Jin, Author
Georg, Ina, Author
Scherthan, Harry1, Author              
Merkenschlager, Matthias, Author
Guillou, Florian, Author
Scherer, Gerd, Author
Barrionuevo, Francisco, Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: Dicer; miRNAs; Testis; Spermatogenesis; Sterility
 Abstract: Dicer is a key enzyme that processes microRNA precursors into their mature form, enabling them to regulate gene expression. Dicer null mutants die before gastrulation. To study Dicer function in testis development, we crossed mice carrying a conditional Dicer allele with an AMH-Cre transgenic line, thereby inactivating Dicer in Sertoli cells around embryonic day 14.0 (E14.0). Dicer null Sertoli cells show normal embryonic development, and at postnatal day 0 (P0), testis tubules are normal in number and histologically undistinguishable from controls. Subsequently, Dicer-mutant testes show a progressively aberrant development, so that at P6, they contain a reduced number of disorganized testis tubules leading to primary sterility. Apoptosis and prophase I assays reveal a massive wave of apoptosis starting at P3, causing progressive loss of Sertoli cells, but also of germ cells, resulting in drastically reduced testis size. Expression of genes that play crucial roles in testis development, structural integrity and spermatogenesis is downregulated at P0, before morphological changes become apparent, indicating that Dicer-mutant testes are already transcriptionally compromised at this stage. Taken together, the results of this study show that Dicer is required for Sertoli cell function and survival and for spermatogenesis in mice.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 472721
DOI: 10.1387/ijdb.092874gk
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Title: International Journal of Developmental Biology
  Alternative Title : Int J Dev Biol
Source Genre: Journal
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Pages: - Volume / Issue: 54 Sequence Number: - Start / End Page: 867 - 875 Identifier: ISSN: 0214-6282