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  An ES-like pluripotent state in FGF-dependent murine iPS cells

Di Stefano, B., Buecker, C., Ungaro, F., Prigione, A., Chen, H. H., Welling, M., et al. (2010). An ES-like pluripotent state in FGF-dependent murine iPS cells. PLoS ONE, 5(12), e16092-e16092. doi:10.1371/journal.pone.0016092.

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Di Stefano, B., Author
Buecker, C., Author
Ungaro, F., Author
Prigione, A.1, Author              
Chen, H. H., Author
Welling, M., Author
Eijpe, M., Author
Mostoslavsky, G., Author
Tesar, P., Author
Adjaye, J.1, Author              
Geijsen, N., Author
Broccoli, V., Author
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              

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 Abstract: Recent data demonstrates that stem cells can exist in two morphologically, molecularly and functionally distinct pluripotent states; a naive LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naive ES-like/ICM properties. FGF-iPSCs display X-chromosome activation, multi-lineage differentiation, teratoma competence and chimera contribution in vivo. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. Characterization of the key molecular signalling pathways revealed FGF-iPSCs to depend on the Activin/Nodal and FGF pathways, while signalling through the JAK-STAT pathway is not required for FGF-iPS cell maintenance. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
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 Identifiers: eDoc: 552361
DOI: 10.1371/journal.pone.0016092
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Title: PLoS ONE
Source Genre: Journal
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Pages: - Volume / Issue: 5 (12) Sequence Number: - Start / End Page: e16092 - e16092 Identifier: ISSN: 1932-6203 (Electronic)