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  Structure of Monomeric Yeast and Mammalian Sec61 Complexes Interacting with the Translating Ribosome.

Becker, T., Bhushan, S., Jarasch, A., Armache, J.-P., Soledad, S., Jossinet, F., et al. (2009). Structure of Monomeric Yeast and Mammalian Sec61 Complexes Interacting with the Translating Ribosome. Science, 326(5958), 1369-1373. doi:10.1126/science.1178535.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7CB0-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7CB1-F
Genre: Journal Article
Alternative Title : Science

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 Creators:
Becker, Thomas, Author
Bhushan, Shashi, Author
Jarasch, Alexander, Author
Armache, Jean-Paul, Author
Soledad, Soledad, Author
Jossinet, Fabrice, Author
Gumbart, James, Author
Mielke, Thorsten1, Author              
Berninghausen, Otto, Author
Schulten, Klaus, Author
Westhof, Eric, Author
Gilmore, Reid, Author
Mandon, Elisabet C., Author
Beckmann, Roland, Author
Affiliations:
1Imaging/Electron Microscopy (Head: Rudi Lurz/Thorsten Mielke), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              

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 Abstract: The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo–electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation.

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Language(s): eng - English
 Dates: 2009-12-04
 Publication Status: Published in print
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Title: Science
  Alternative Title : Science
Source Genre: Journal
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Pages: - Volume / Issue: 326 (5958) Sequence Number: - Start / End Page: 1369 - 1373 Identifier: ISSN: 0036-8075