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  Stress response in tardigrades: differential gene expression of molecular chaperones.

Reuner, A., Hengherr, S., Mali, B., Förster, F., Arndt, D., Reinhardt, R., et al. (2009). Stress response in tardigrades: differential gene expression of molecular chaperones. Cell Stress and Chaperones, epub-epub. doi:10.1007/s12192-009-0158-1.

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Genre: Journal Article
Alternative Title : Cell Stress Chaperones

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 Creators:
Reuner, Andy, Author
Hengherr, Steffen, Author
Mali, Brahim, Author
Förster, Frank, Author
Arndt, Detlev, Author
Reinhardt, Richard1, Author              
Dandekar, Thomas, Author
Frohme, Marcus, Author
Brümmer, Franz, Author
Schill, Ralph O., Author
Affiliations:
1High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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Free keywords: Alpha-crystallin protein; Anhydrobiosis; Cryptobiosis; Heat-shock protein; Tardigrada; Milnesium tardigradum
 Abstract: Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (Mt-sHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.

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Language(s): eng - English
 Dates: 2009-11-27
 Publication Status: Published in print
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Title: Cell Stress and Chaperones
  Alternative Title : Cell Stress Chaperones
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: epub - epub Identifier: ISSN: 1355-8145