English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  DNA methylation in the IGF2 intragenic DMR is re-established in a sex-specific manner in bovine blastocysts after somatic cloning.

Geberta, C., Wrenzyckia, C., Herrmann, D., Gröger, D., Thiel, J., Reinhardt, R., et al. (2009). DNA methylation in the IGF2 intragenic DMR is re-established in a sex-specific manner in bovine blastocysts after somatic cloning. Genomics, 94(1), 63-69. Retrieved from http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WG1-4VYP93F-1&_user=28761&_coverDate=07%2F31%2F2009&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000002718&_version=1&_urlVersion=0&_userid=28761&md5=66994da7b0000fca304cfe9cd5576a1c.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : Genomics

Files

show Files
hide Files
:
63.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
63.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Molecular Genetics, MBMG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: MPG
License:
-

Locators

show

Creators

show
hide
 Creators:
Geberta, Claudia, Author
Wrenzyckia, Christine, Author
Herrmann, Doris, Author
Gröger, Daniela1, Author
Thiel, Janina1, Author
Reinhardt, Richard2, Author              
Lehrach, Hans3, Author              
Hajkova, Petra, Author
Lucas-Hahn, Andrea, Author
Carnwath, Joseph W., Author
Niemann, Heiner, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Content

show
hide
Free keywords: Genomic imprinting; Somatic cloning; Bovine embryos; Sex-specific DNA methylation; Epigenetic reprogramming
 Abstract: The recent identification of an intragenic differentially methylated region (DMR) within the last exon of the bovine Insulin-like growth factor 2 (IGF2) gene provides a diagnostic tool for in-depth investigation of bovine imprinting and regulatory mechanisms which are active during embryo development. Here, we used bisulfite sequencing to compare sex-specific DNA methylation patterns within this DMR in bovine blastocysts produced in vivo, by in vitro fertilization and culture, SCNT, androgenesis or parthenogenesis. In in vivo derived embryos, DNA methylation was removed from this intragenic DMR after fertilization, but partially replaced by the time the embryo reached the blastocyst stage. Among embryos developing in vivo, the level of DNA methylation was significantly lower in female than in male blastocysts. This sexual dimorphism was also found between parthenogenetic and androgenetic embryos, and followed the donor cell sex in SCNT derived blastocysts and is evidence for correct methylation reprogramming in SCNT embryos.

Details

show
hide
Language(s): eng - English
 Dates: 2009-10-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genomics
  Alternative Title : Genomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 94 (1) Sequence Number: - Start / End Page: 63 - 69 Identifier: ISSN: 0888-7543