English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression

Stoeckius, M., Maaskola, J., Colombo, T., Rahn, H.-P., Friedländer, M. R., Li, N., et al. (2009). Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression. Nature Methods, 6, 745-751. doi:10.1038/nmeth.1370.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D13-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D14-B
Genre: Journal Article
Alternative Title : Nat Mathods

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Stoeckius, Marlon, Author
Maaskola, Jonas, Author
Colombo, Teresa, Author
Rahn, Hans-Peter, Author
Friedländer, Marc R., Author
Li, Na1, Author              
Chen, Wei2, Author              
Piano, Fabio, Author
Rajewsky, Nikolaus, Author
Affiliations:
1Computational Epigenetics (Ho-Ryun Chung), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479658              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

Content

show
hide
Free keywords: -
 Abstract: Caenorhabditis elegans is one of the most prominent model systems for embryogenesis, but collecting many precisely staged embryos has been impractical. Thus, early C. elegans embryogenesis has not been amenable to most high-throughput genomics or biochemistry assays. To overcome this problem, we devised a method to collect staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). In a proof-of-principle experiment, we found that a single eFACS run routinely yielded tens of thousands of almost perfectly staged 1-cell stage embryos. As the earliest embryonic events are driven by posttranscriptional regulation, we combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We discovered complex and orchestrated changes in the expression between and within almost all classes of small RNAs, including microRNAs and 26G-RNAs, during embryogenesis.

Details

show
hide
Language(s): eng - English
 Dates: 2009-09-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Methods
  Alternative Title : Nat Mathods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 6 Sequence Number: - Start / End Page: 745 - 751 Identifier: ISSN: 1548-7091