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  Designing evolvable libraries using multi-body potentials

Lappe, M., Bagler, G., Filippis, I., Stehr, H., Duarte, J. M., & Sathyapriya, R. (2009). Designing evolvable libraries using multi-body potentials. Current Opinion in Biotechnology, 24(4), 437-446. doi:10.1016/j.copbio.2009.07.008.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D46-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D47-8
Genre: Journal Article
Alternative Title : Curr Opin Biotechnol

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 Creators:
Lappe, Michael1, Author              
Bagler, Ganesh1, Author              
Filippis, Ioannis2, Author
Stehr, Henning1, Author              
Duarte, Jose M.1, Author              
Sathyapriya, Rajagopal1, Author              
Affiliations:
1Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433554              
2Max Planck Society, ou_persistent13              

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 Abstract: Novel high-throughput technologies for directed evolution enable experimental coverage of an impressive number of sequences. Nevertheless, the success of such experiments hinges on the initial sequence libraries. Here we consider the computational design of smart focused libraries and review insights from experimental strategies and theoretic advances in modelling their energy landscapes. In library design as in structure prediction, the applied energy function is the key. Current knowledge-based potentials have proven more successful than purely physics-based ones. Here we summarize novel approaches that extend the classical pairwise treatment of residue contacts towards adaptive knowledge-based multi-body potentials. We suggest that minimal sets of probabilistic constraints will lead to much more efficient sampling of permissible conformations and sequence space.

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Language(s): eng - English
 Dates: 2009-08
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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Title: Current Opinion in Biotechnology
  Alternative Title : Curr Opin Biotechnol
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 24 (4) Sequence Number: - Start / End Page: 437 - 446 Identifier: ISSN: 0958-1669