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  The Spt-Ada-Gcn5-acetyltransferase complex interaction motif of E2a is essential for a subset of transcriptional and oncogenic properties of E2a-Pbx1

Scheele, J. S., Kolanczyk, M., Gantert, M., Zemojtel, T., Dorn, A., Sykes, D. B., et al. (2009). The Spt-Ada-Gcn5-acetyltransferase complex interaction motif of E2a is essential for a subset of transcriptional and oncogenic properties of E2a-Pbx1. Leukemia & Lymphoma, 50(5), 816-828. doi:10.1080/10428190902836107.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D9C-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D9D-8
Genre: Journal Article
Alternative Title : Leuk Lymphoma

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 Creators:
Scheele, Jürgen S., Author
Kolanczyk, Mateusz1, Author              
Gantert, Melanie, Author
Zemojtel, Tomasz2, Author              
Dorn, Annette, Author
Sykes, David B., Author
Möbest, Dietrich C. C., Author
Kamps, Mark P., Author
Räpple, Daniel, Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              
2Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              

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 Abstract: The oncogene E2a-Pbx1 is formed by the t(1;19) translocation, which joins the N-terminal transactivation domain of E2a with the C-terminal homeodomain of PBX1. The goal of this work was to elucidate the mechanisms by which E2a-Pbx1 can lead to deregulated target gene expression. For reporter constructs it was shown that E2a-Pbx1 can activate transcription through homodimer elements (TGATTGAT) or through heterodimer elements with Hox proteins (e.g. TGATTAAT). We show a novel mechanism by which E2a-Pbx1 activates transcription of EF-9 using a promoter in intron 1 of the EF-9 gene, resulting in an aminoterminal truncated transcript. Our results indicate that the LDFS motif of E2a is essential for the transactivation of EF-9, but dispensable for transactivation of fibroblast growth factor 15. The E2a LDFS motif was also essential for proliferation of NIH3T3 fibroblasts but was dispensable for the E2a-Pbx1-induced differentiation arrest of myeloid progenitors.

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Language(s): eng - English
 Dates: 2009-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 447281
DOI: 10.1080/10428190902836107
 Degree: -

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Title: Leukemia & Lymphoma
  Alternative Title : Leuk Lymphoma
Source Genre: Journal
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Pages: - Volume / Issue: 50 (5) Sequence Number: - Start / End Page: 816 - 828 Identifier: ISSN: 1042-8194