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  PBX1 is dispensable for neural commitment of RA-treated murine ES cells

Jürgens, A. S., Kolanczyk, M., Moebest, D. C. C., Zemojtel, T., Lichtenauer, U., Duchniewicz, M., et al. (2009). PBX1 is dispensable for neural commitment of RA-treated murine ES cells. In Vitro Cellular & Developmental Biology - Animal, 45(5-6), 252-263. doi:10.1007/s11626-008-9162-5.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D9E-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7D9F-4
Genre: Journal Article
Alternative Title : In Vitro Cell Dev Biol Anim

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 Creators:
Jürgens, Anne S., Author
Kolanczyk, Mateusz1, Author              
Moebest, Dietrich C. C., Author
Zemojtel, Tomasz2, Author              
Lichtenauer, Urs, Author
Duchniewicz, Marlena, Author
Gantert, Melanie P., Author
Hecht, Jochen1, Author              
Hattenhorst, Uwe, Author
Burdach, Stefan, Author
Dorn, Annette, Author
Kamps, Mark P., Author
Beuschlein, Felix, Author
Räpple, Daniel, Author
Scheele, Jürgen S., Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              
2Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              

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Free keywords: PBX1; Differentiation; ES cells; Imprinting; Expression profiling
 Abstract: Experimentation with PBX1 knockout mice has shown that PBX1 is necessary for early embryogenesis. Despite broad insight into PBX1 function, little is known about the underlying target gene regulation. Utilizing the Cre–loxP system, we targeted a functionally important part of the homeodomain of PBX1 through homozygous deletion of exon-6 and flanking intronic regions leading to exon 7 skipping in embryonic stem (ES) cells. We induced in vitro differentiation of wild-type and PBX1 mutant ES cells by aggregation and retinoic acid (RA) treatment and compared their profiles of gene expression at the ninth day post-reattachment to adhesive media. Our results indicate that PBX1 interactions with HOX proteins and DNA are dispensable for RA-induced ability of ES to express neural genes and point to a possible involvement of PBX1 in the regulation of imprinted genes.

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Language(s): eng - English
 Dates: 2009-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

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Title: In Vitro Cellular & Developmental Biology - Animal
  Alternative Title : In Vitro Cell Dev Biol Anim
Source Genre: Journal
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Pages: - Volume / Issue: 45 (5-6) Sequence Number: - Start / End Page: 252 - 263 Identifier: ISSN: 1071-2690