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  Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments.

Jogler, C., Lin, W., Meyerdierks, A., Kube, M., Katzmann, E., Flies, C., et al. (2009). Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments. Applied and Environmental Microbiology, 75(12), 3972-3979. doi:10.1128/AEM.02701-08.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7DAB-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7DAC-8
Genre: Journal Article
Alternative Title : Appl. Environ. Microbiol.

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 Creators:
Jogler, Christian, Author
Lin, Wei, Author
Meyerdierks, Anke1, Author
Kube, Michael2, Author              
Katzmann, Emanuel, Author
Flies, Christine1, Author
Pan, Yongxin, Author
Amann, Rudolf1, Author
Reinhard, Richard1, Author
Schüler, Dirk2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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 Abstract: In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fosmid libraries were constructed and screened by end sequencing and hybridization analysis using heterologous magnetosome gene probes. A total of 14 fosmids were fully sequenced. We identified and characterized two fosmids, most likely originating from two different alphaproteobacterial strains of MTB that contain several putative operons with homology to the magnetosome island (MAI) of cultivated MTB. This is the first evidence that uncultivated MTB exhibit similar yet differing organizations of the MAI, which may account for the diversity in biomineralization and magnetotaxis observed in MTB from various environments.

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Language(s): eng - English
 Dates: 2009-04-24
 Publication Status: Published in print
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Title: Applied and Environmental Microbiology
  Alternative Title : Appl. Environ. Microbiol.
Source Genre: Journal
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Pages: - Volume / Issue: 75 (12) Sequence Number: - Start / End Page: 3972 - 3979 Identifier: ISSN: 0099-2240