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  Phylogenetic classification and identification of bacteria by mass spectrometry

Freiwald, A., & Sauer, S. (2009). Phylogenetic classification and identification of bacteria by mass spectrometry. Nature Protocols, 4(5), 732-742. doi:10.1038/nprot.2009.37.

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 Creators:
Freiwald, Anja1, Author              
Sauer, Sascha1, Author              
Affiliations:
1Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479662              

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 Abstract: Bacteria are a convenient source of intrinsic marker proteins, which can be detected efficiently by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The patterns of protein masses observed can be used for accurate classification and identification of bacteria. Key to the reliability of the method is a robust and standardized procedure for sample preparations, including bacterial culturing, chemical treatment for bacterial cell wall disruption and for protein extraction, and mass spectrometry analysis. The protocol is an excellent alternative to classical microbiological classification and identification procedures, requiring minimal sample preparation efforts and costs. Without cell culturing, the protocol takes in general <1 h.

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Language(s): eng - English
 Dates: 2009-04-23
 Publication Status: Published in print
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Title: Nature Protocols
Source Genre: Journal
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Pages: - Volume / Issue: 4 (5) Sequence Number: - Start / End Page: 732 - 742 Identifier: ISSN: 1754-2189