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  An empirical framework for binary interactome mapping

Venkatesan, K., Rual, J.-F., Vazquez, A., Stelzl, U., Lemmens, I., Hirozane-Kishikawa, T., et al. (2009). An empirical framework for binary interactome mapping. Nature Methods, 6(1), 83-90. doi:10.1038/nmeth.1280.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7E4B-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7E4C-1
Genre: Journal Article

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 Creators:
Venkatesan, Kavitha, Author
Rual, Jean-François, Author
Vazquez, Alexei, Author
Stelzl, Ulrich1, Author              
Lemmens, Irma, Author
Hirozane-Kishikawa, Tomoko, Author
Hao, Tong, Author
Zenkner, Martina, Author
Xin, Xiaofeng, Author
Goh, Kwang-Il., Author
Yildirim, Muhammed A., Author
Simonis, Nicolas, Author
Heinzmann, Kathrin, Author
Gebreab, Fana, Author
Sahalie, Julie M., Author
Cevik, Sebiha, Author
Simon, Christophe, Author
de Smet, Anne-Sophie, Author
Dann, Elizabeth, Author
Smolyar, Alex, Author
Vinayagam, Arunachalam, AuthorYu, Haiyuan, AuthorSzeto, David, AuthorBorick, Heather, AuthorDricot, Amélie, AuthorKlitgord, Niels, AuthorMurray, Ryan R., AuthorLin, Chenwei, AuthorLalowski, Maciej, AuthorTimm, Jan, AuthorRau, Kirstin, AuthorBoone, Charles, AuthorBraun, Pascal, AuthorCusick, Michael E., AuthorRoth, Frederick P., AuthorHill, David E., AuthorTavernier, Jan, AuthorWanker, Erich E., AuthorBarabási, Albert-László, AuthorVidal, Marc, Author more..
Affiliations:
1Molecular Interaction Networks (Ulrich Stelzl), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479660              

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 Abstract: Several attempts have been made to systematically map protein-protein interaction, or 'interactome', networks. However, it remains difficult to assess the quality and coverage of existing data sets. Here we describe a framework that uses an empirically-based approach to rigorously dissect quality parameters of currently available human interactome maps. Our results indicate that high-throughput yeast two-hybrid (HT-Y2H) interactions for human proteins are more precise than literature-curated interactions supported by a single publication, suggesting that HT-Y2H is suitable to map a significant portion of the human interactome. We estimate that the human interactome contains approx130,000 binary interactions, most of which remain to be mapped. Similar to estimates of DNA sequence data quality and genome size early in the Human Genome Project, estimates of protein interaction data quality and interactome size are crucial to establish the magnitude of the task of comprehensive human interactome mapping and to elucidate a path toward this goal.

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Language(s): eng - English
 Dates: 2009-01
 Publication Status: Published in print
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Title: Nature Methods
Source Genre: Journal
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Pages: - Volume / Issue: 6 (1) Sequence Number: - Start / End Page: 83 - 90 Identifier: ISSN: 1548-7091