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Fluorescence microscopy, Green fluorescent proteins; Protein folding; Ribosomes; Single-molecule studies
Abstract:
It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute.