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  A human snoRNA with microRNA-like functions

Ender, C., Krek, A., Friedländer, M. R., Beitzinger, M., Weinmann, L., Chen, W., et al. (2008). A human snoRNA with microRNA-like functions. Molecular Cell, 32(4), 519-528. doi:10.1016/j.molcel.2008.10.017.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7EAC-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7EAD-9
Genre: Journal Article
Alternative Title : Mol Cell

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 Creators:
Ender, Christine1, Author
Krek, Azra, Author
Friedländer, Marc R., Author
Beitzinger, Michaela, Author
Weinmann, Lasse, Author
Chen, Wei2, Author              
Pfeffer, Sébastien, Author
Rajewsky, Nikolaus, Author
Meister, Gunter, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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 Abstract: Small noncoding RNAs function in concert with Argonaute (Ago) proteins to regulate gene expression at the level of transcription, mRNA stability, or translation. Ago proteins bind small RNAs and form the core of silencing complexes. Here, we report the analysis of small RNAs associated with human Ago1 and Ago2 revealed by immunoprecipitation and deep sequencing. Among the reads, we find small RNAs originating from the small nucleolar RNA (snoRNA) ACA45. Moreover, processing of ACA45 requires Dicer activity but is independent of Drosha/DGCR8. Using bioinformatic prediction algorithms and luciferase reporter assays, we uncover the mediator subunit CDC2L6 as one potential mRNA target of ACA45 small RNAs, suggesting a role for ACA45-processing products in posttranscriptional gene silencing. We further identify a number of human snoRNAs with microRNA (miRNA)-like processing signatures. We have, therefore, identified a class of small RNAs in human cells that originate from snoRNAs and can function like miRNAs.

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Language(s): eng - English
 Dates: 2008-11-21
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 407116
URI: 10.1016/j.molcel.2008.10.017
DOI: 10.1016/j.molcel.2008.10.017
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Title: Molecular Cell
  Alternative Title : Mol Cell
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 32 (4) Sequence Number: - Start / End Page: 519 - 528 Identifier: ISSN: 1097-2765