English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Ca 2+ signaling occurs via second messenger release from intraorganelle synthesis sites

Davis, L. C., Morgan, A. J., Ruas, M., Wong, J. L., Graef, R. M., Poustka, A. J., et al. (2008). Ca 2+ signaling occurs via second messenger release from intraorganelle synthesis sites. Current Biology, 18(20), 1612-1618. doi:10.1016/j.cub.2008.09.024.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7EC5-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7EC6-F
Genre: Journal Article
Alternative Title : Curr Biol

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Davis, Lianne C., Author
Morgan, Anthony J., Author
Ruas, Margarida, Author
Wong, Julian L., Author
Graef, Richard M., Author
Poustka, Albert J.1, Author              
Lee, Hon Cheung, Author
Wessel, Gary M., Author
Parrington, John, Author
Galione, Antony, Author
Affiliations:
1Evolution and Development (Albert Poustka), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479650              

Content

show
hide
Free keywords: Signaling
 Abstract: Cyclic ADP-ribose is an important Ca2+-mobilizing cytosolic messenger synthesized from β-NAD+ by ADP-ribosyl cyclases (ARCs). However, the focus upon ectocellular mammalian ARCs (CD38 and CD157) has led to confusion as to how extracellular enzymes generate intracellular messengers in response to stimuli. We have cloned and characterized three ARCs in the sea urchin egg and found that endogenous ARCβ and ARCγ are intracellular and located within the lumen of acidic, exocytotic vesicles, where they are optimally active. Intraorganelle ARCs are shielded from cytosolic substrate and targets by the organelle membrane, but this barrier is circumvented by nucleotide transport. We show that a β-NAD+ transporter provides ARC substrate that is converted luminally to cADPR, which, in turn, is shuttled out to the cytosol via a separate cADPR transporter. Moreover, nucleotide transport is integral to ARC activity physiologically because three transport inhibitors all inhibited the fertilization-induced Ca2+ wave that is dependent upon cADPR. This represents a novel signaling mechanism whereby an extracellular stimulus increases the concentration of a second messenger by promoting messenger transport from intraorganelle synthesis sites to the cytosol.

Details

show
hide
Language(s): eng - English
 Dates: 2008-10-28
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Current Biology
  Alternative Title : Curr Biol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 18 (20) Sequence Number: - Start / End Page: 1612 - 1618 Identifier: ISSN: 0960-9822