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  In vitro selection of random RNA fragments to identify protein-binding sites within large RNAs

Stelz, U., & Nierhaus, K. (2008). In vitro selection of random RNA fragments to identify protein-binding sites within large RNAs. In R.-J. Lin (Ed.), RNA-Protein Interaction Protocols (pp. 247-255). Berlin [et al]: Springer.

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10.1007_978-1-60327-475-3@17.pdf (Any fulltext), 443KB
 
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 Creators:
Stelz, Ulrich1, Author
Nierhaus, Knud2, Author           
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1Max Planck Society, ou_persistent13              
2Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433558              

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Free keywords: In vitro selection - ribosome - RNA binding site - RNA-protein interaction - SELEX - SERF
 Abstract: In vitro selection experiments have various goals depending on the composition of the initial pool and the selection method applied. We developed an in vitro selection variant that is useful for the identification of minimal RNA binding sites for proteins within large RNAs. A pool of randomly fragmented RNA is constructed from a large RNA, which is the natural binding partner for a protein. Such a pool contains all the potential binding sites and is therefore used as starting material for affinity selection. A successful in vitro selection with the purified protein will identify the protein's natural RNA target site. The method has been developed for ribosomal systems and is a general approach providing a basis for the functional and structural characterization of large ribonucleoprotein particles.

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Language(s): eng - English
 Dates: 2008-10-01
 Publication Status: Published in print
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Title: RNA-Protein Interaction Protocols
Source Genre: Book
 Creator(s):
Lin, Ren-Jang, Editor
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Publ. Info: Berlin [et al] : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 247 - 255 Identifier: ISBN: 978-1-58829-419-7

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 488 Sequence Number: - Start / End Page: - Identifier: -