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  A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome

Sultan, M., Schulz, M. H., Hugues, R., Magen, A., Klingenhoff, A., Scherf, M., et al. (2008). A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome. Science, 321(5891), 956-960. doi:10.1126/science.1160342.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7F47-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7F48-1
Genre: Journal Article
Alternative Title : Scie

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 Creators:
Sultan, Marc1, Author              
Schulz, Marcel H.2, Author
Hugues, Richard2, Author
Magen, Alon2, Author
Klingenhoff, Andreas, Author
Scherf, Matthias, Author
Seifert, Martin, Author
Borodina, Tatjana3, Author              
Soldatov, Aleksey3, Author              
i Parkhomchuk, Dmitr2, Author
Schmidt, Dominic2, Author
O'Keeffe, Sean2, Author
Haas, Stefan4, Author              
Vingron, Martin5, Author              
Lehrach, Hans6, Author              
Yaspo, Marie-Laure1, Author              
Affiliations:
1Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              
2Max Planck Society, ou_persistent13              
3Technology Development(Alexey Soldatov), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479657              
4Gene Structure and Array Design (Stefan Haas), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479640              
5Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              
6Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Abstract: The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney and a B cell line. We used shotgun sequencing of transcripts to generate randomly distributed reads. Of these, 50% mapped to unique genomic locations, of which 80% corresponded to known exons. We found that 66% of the polyadenylated transcriptome mapped to known genes and 34% to nonannotated genomic regions. On the basis of known transcripts, RNA-Seq can detect 25% more genes than can microarrays. A global survey of messenger RNA splicing events identified 94,241 splice junctions (4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing.

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Language(s): eng - English
 Dates: 2008-08-15
 Publication Status: Published in print
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Title: Science
  Alternative Title : Scie
Source Genre: Journal
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Pages: - Volume / Issue: 321 (5891) Sequence Number: - Start / End Page: 956 - 960 Identifier: ISSN: 0036-8075