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  A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome

Sultan, M., Schulz, M. H., Hugues, R., Magen, A., Klingenhoff, A., Scherf, M., et al. (2008). A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome. Science, 321(5891), 956-960. doi:10.1126/science.1160342.

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Genre: Zeitschriftenartikel
Alternativer Titel : Scie

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 Urheber:
Sultan, Marc1, Autor           
Schulz, Marcel H.2, Autor
Hugues, Richard2, Autor
Magen, Alon2, Autor
Klingenhoff, Andreas, Autor
Scherf, Matthias, Autor
Seifert, Martin, Autor
Borodina, Tatjana3, Autor           
Soldatov, Aleksey3, Autor           
i Parkhomchuk, Dmitr2, Autor
Schmidt, Dominic2, Autor
O'Keeffe, Sean2, Autor
Haas, Stefan4, Autor           
Vingron, Martin5, Autor           
Lehrach, Hans6, Autor           
Yaspo, Marie-Laure1, Autor           
Affiliations:
1Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              
2Max Planck Society, ou_persistent13              
3Technology Development(Alexey Soldatov), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479657              
4Gene Structure and Array Design (Stefan Haas), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479640              
5Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              
6Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Zusammenfassung: The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney and a B cell line. We used shotgun sequencing of transcripts to generate randomly distributed reads. Of these, 50% mapped to unique genomic locations, of which 80% corresponded to known exons. We found that 66% of the polyadenylated transcriptome mapped to known genes and 34% to nonannotated genomic regions. On the basis of known transcripts, RNA-Seq can detect 25% more genes than can microarrays. A global survey of messenger RNA splicing events identified 94,241 splice junctions (4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing.

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Sprache(n): eng - English
 Datum: 2008-08-15
 Publikationsstatus: Erschienen
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Titel: Science
  Alternativer Titel : Scie
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 321 (5891) Artikelnummer: - Start- / Endseite: 956 - 960 Identifikator: ISSN: 0036-8075