English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain

Jeong, J.-H., Jin, J.-S., Kim, H.-N., Kang, S.-M., Liu, J. C., Lengner, C. J., et al. (2008). Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain. Journal of Cellular Physiology, 217(2), 511-517. doi:10.1002/jcp.21524.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7F7D-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7F7E-B
Genre: Journal Article
Alternative Title : J Cell Physiol.

Files

show Files
hide Files
:
fulltext.pdf (Any fulltext), 302KB
 
File Permalink:
-
Name:
fulltext.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Molecular Genetics, Berlin; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: MPG
License:
-

Locators

show

Creators

show
hide
 Creators:
Jeong, Jae-Hwan, Author
Jin, Jung-Sook, Author
Kim, Hyun-Nam, Author
Kang, Sang-Min, Author
Liu, Julie C., Author
Lengner, Christopher J., Author
Otto, Florian, Author
Mundlos, Stefan1, Author              
Stein, Janet L., Author
van Wijnen, Andre J., Author
Lian, Jane B., Author
Stein, Gary S., Author
Choi, Je-Yong, Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              

Content

show
hide
Free keywords: -
 Abstract: Runx2 is a master transcription factor for chondrocyte and osteoblast differentiation and bone formation. However expression of Runx2 (by RT-PCR), has been reported in non-skeletal tissues such as breast, T cells and testis. To better define Runx2 activity in non-skeletal tissues, we examined transgenic (Tg) mice expressing LacZ gene under control of 3.0 kb (3 kb Tg) or 1.0 kb (1 kb Tg) of the Runx2 distal (P1) promoter, Runx2 LacZ knock-in (Runx2+/LacZ) and Runx2/P1 LacZ knock-in (Runx2/P1+/LacZ). In the Runx2 3 kb Tg mouse, -galactosidase (-gal) expression appeared in various non-skeletal tissues including testis, skin, adrenal gland and brain. -gal expression from both 3 kb and 1 kb Tg, reflecting activity of the Runx2 promoter, was readily detectable in seminiferous tubules of the testis and the epididymis. At the single cell level, -gal was detected in spermatids and mature sperms not in sertoli or Leydig cells. We also detected a positive signal from the Runx2+/LacZ and Runx2/P1+/LacZ mice. Indeed, Runx2 expression was observed in isolated mature sperms, which was confirmed by RT-PCR and Western blot analysis. Runx2, however, was not related to sex determination and sperm motility. Runx2 mediated -gal activity is also found robustly in the hippocampus and frontal lobe of the brain in Runx2+/LacZ. Collectively, these results indicate that Runx2 is expressed in several non-skeletal tissues particularly sperms of testis and hippocampus of brain. It suggests that Runx2 may play an important role in male reproductive organ testis and brain.

Details

show
hide
Language(s): eng - English
 Dates: 2008-07-17
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Cellular Physiology
  Alternative Title : J Cell Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 217 (2) Sequence Number: - Start / End Page: 511 - 517 Identifier: -