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  Shine-Dalgarno interaction prevents incorporation of noncognate amino acids at the codon following the AUG

Di Giacco, V., Márquez, V., Yan Qin, Y., Pech, M., Triana-Alonso, F. J., Wilson, D. N., et al. (2008). Shine-Dalgarno interaction prevents incorporation of noncognate amino acids at the codon following the AUG. Proceedings of the National Academy of Sciences of the United States of America, 105(31), 10715-10720. Retrieved from http://www.pnas.org/content/105/31/10715.full.pdf+html.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7FCE-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-429A-4
Genre: Journal Article
Alternative Title : Proc Natl Acad Sci U S A.

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Di Giacco, Viviana, Author
Márquez, Viter, Author
Yan Qin, Yan, Author
Pech, Markus1, Author              
Triana-Alonso, Francisco J., Author
Wilson, Daniel N.2, Author              
Nierhaus, Knud H.3, Author              
Affiliations:
1Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
3Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433558              

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 Abstract: During translation, usually only one in approximately 400 misincorporations affects the function of a nascent protein, because only chemically similar near-cognate amino acids are misincorporated in place of the cognate one. The deleterious misincorporation of a chemically dissimilar noncognate amino acid during the selection process is precluded by the presence of a tRNA at the ribosomal E-site. However, the selection of first aminoacyl-tRNA, directly after initiation, occurs without an occupied E-site, i.e., when only the P-site is filled with the initiator tRNA and thus should be highly error-prone. Here, we show how bacterial ribosomes have solved this accuracy problem: In the absence of a Shine-Dalgarno (SD) sequence, the first decoding step at the A-site after initiation is extremely error-prone, even resulting in the significant incorporation of noncognate amino acids. In contrast, when a SD sequence is present, the incorporation of noncognate amino acids is not observed. This is precisely the effect that the presence of a cognate tRNA at the E-site has during the elongation phase. These findings suggest that during the initiation phase, the SD interaction functionally compensates for the lack of codon-anticodon interaction at the E-site by reducing the misincorporation of near-cognate amino acids and prevents noncognate misincorporation.

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Language(s): eng - English
 Dates: 2008-05-27
 Publication Status: Published in print
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Alternative Title : Proc Natl Acad Sci U S A.
Source Genre: Journal
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Pages: - Volume / Issue: 105 (31) Sequence Number: - Start / End Page: 10715 - 10720 Identifier: -