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  Increasing genomic information in bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution

Tanguy, A., Bierne, N., Saavedra, C., Pina, B., Bachère, E., Kube, M., et al. (2008). Increasing genomic information in bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution. Gene, 408(1-2), 27-36. doi:10.1016/j.gene.2007.10.021.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8074-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8075-3
Genre: Journal Article
Alternative Title : Gene

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 Creators:
Tanguy, Arnaud, Author
Bierne, Nicolas, Author
Saavedra, Carlos, Author
Pina, Benjamin, Author
Bachère, Evelyne, Author
Kube, Michael1, Author              
Bazin, Eric, Author
Bonhomme, François, Author
Boudry, Pierre, Author
Boulo, Viviane, Author
Boutet, Isabelle, Author
Cancela, Leonor, Author
Dossat, Carole, Author
Favrel, Pascal, Author
Huvet, Arnaud, Author
Jarque, Sergio, Author
Jollivet, Didier, Author
Klages, Sven2, Author              
Lapègue, Sylvie, Author
Leite, Ricardo, Author
Moal, Jeanne, AuthorMoraga, Dario, AuthorReinhardt, Richard1, Author              Samain, Jean-François, AuthorZouros, Eleftherios, AuthorCanario, Adelino, Author more..
Affiliations:
1High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Molluscs; ESTs; Environment; Evolution
 Abstract: The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300–3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps.

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Language(s): eng - English
 Dates: 2008-01-31
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

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Title: Gene
  Alternative Title : Gene
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 408 (1-2) Sequence Number: - Start / End Page: 27 - 36 Identifier: ISSN: 0378-1119