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  Comparative analysis of expressed sequence tag (EST) libraries in the seagrass Zostera marina subjected to temperature stress

Reusch, T. B. H., Veron, A. S., Preuss, C., Weiner, J., Wissler, L., Beck, A., et al. (2008). Comparative analysis of expressed sequence tag (EST) libraries in the seagrass Zostera marina subjected to temperature stress. Marine Biotechnology, 10(3), 297-309. doi:10.1007/s10126-007-9065-6.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8087-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8088-A
Genre: Journal Article
Alternative Title : Mar Biotechnol (NY)

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 Creators:
Reusch, Thorsten B. H., Author
Veron, Amelie S., Author
Preuss, Christoph, Author
Weiner, January, Author
Wissler, Lothar, Author
Beck, Alfred1, Author              
Klages, Sven2, Author              
Kube, Michael3, Author              
Reinhardt, Richard3, Author              
Bornberg-Bauer, Erich, Author
Affiliations:
1Computing (Head: Donald Buczek/Peter Marquardt), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479667              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
3High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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 Abstract: Global warming is associated with increasing stress and mortality on temperate seagrass beds, in particular during periods of high sea surface temperatures during summer months, adding to existing anthropogenic impacts, such as eutrophication and habitat destruction. We compare several expressed sequence tag (EST) in the ecologically important seagrass Zostera marina (eelgrass) to elucidate the molecular genetic basis of adaptation to environmental extremes. We compared the tentative unigene (TUG) frequencies of libraries derived from leaf and meristematic tissue from a control situation with two experimentally imposed temperature stress conditions and found that TUG composition is markedly different among these conditions (all P < 0.0001). Under heat stress, we find that 63 TUGs are differentially expressed (d.e.) at 25°C compared with lower, no-stress condition temperatures (4°C and 17°C). Approximately one-third of d.e. eelgrass genes were characteristic for the stress response of the terrestrial plant model Arabidopsis thaliana. The changes in gene expression suggest complex photosynthetic adjustments among light-harvesting complexes, reaction center subunits of photosystem I and II, and components of the dark reaction. Heat shock encoding proteins and reactive oxygen scavengers also were identified, but their overall frequency was too low to perform statistical tests. In all conditions, the most abundant transcript (3–15%) was a putative metallothionein gene with unknown function. We also find evidence that heat stress may translate to enhanced infection by protists. A total of 210 TUGs contain one or more microsatellites as potential candidates for gene-linked genetic markers. Data are publicly available in a user-friendly database at http://www.uni-muenster.de/Evolution/ebb/Services/zostera.

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Language(s): eng - English
 Dates: 2008-01-18
 Publication Status: Published in print
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Title: Marine Biotechnology
  Alternative Title : Mar Biotechnol (NY)
Source Genre: Journal
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Pages: - Volume / Issue: 10 (3) Sequence Number: - Start / End Page: 297 - 309 Identifier: ISSN: 1436-2228