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  Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1.

Winter, J., Kunath, M., Roepcke, S., Krause, S., Schneider, R., & Schweiger, S. (2007). Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1. BMC Molecular Biology [Elektronische Ressource], 8(1), 105-133. doi:10.1186/1471-2199-8-105.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8120-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8121-A
Genre: Journal Article

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 Creators:
Winter, Jennifer1, Author              
Kunath, Melanie2, Author
Roepcke, Stefan2, Author
Krause, Sven2, Author
Schneider, Rainer1, Author              
Schweiger, Susann1, Author              
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              
2Max Planck Society, ou_persistent13              

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 Abstract: Background Mutations in the X-linked MID1 gene are responsible for Opitz G/BBB syndrome, a malformation disorder of developing midline structures. Previous Northern blot analyses revealed the existence of at least three MID1 transcripts of differing lengths. Results Here we show that alternative polyadenylation generates the size differences observed in the Northern blot analyses. Analysis of EST data together with additional Northern blot analyses proved tissue-specific usage of the alternative polyadenylation sites. Bioinformatic characterization of the different 3'UTRs of MID1 revealed numerous RNA-protein interaction motifs, several of which turned out to be conserved between different species. Furthermore, our data suggest that mRNA termination at different polyadenylation sites is predetermined by the choice of alternative 5'UTRs and promoters of the MID1 gene, a mechanism that efficiently allows synergistic function of 5' and 3'UTRs. Conclusions MID1 expression is tightly regulated through concerted action of alternative promoters and alternative polyadenylation signals both during embryonic development and in the adult.

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Language(s): eng - English
 Dates: 2007-11-15
 Publication Status: Published in print
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Title: BMC Molecular Biology [Elektronische Ressource]
Source Genre: Journal
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Pages: - Volume / Issue: 8 (1) Sequence Number: - Start / End Page: 105 - 133 Identifier: ISSN: 1471-2199