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  Conserved molecular portraits of bovine and human blastocysts as a consequence of the transition from maternal to embryonic control of gene expression

Adjaye, J., Herwig, R., Brink, T. C., Herrmann, D., Greber, B., Sudheer, S., et al. (2007). Conserved molecular portraits of bovine and human blastocysts as a consequence of the transition from maternal to embryonic control of gene expression. Physiological Genomics, 31(2), 315-327. doi:10.1152/physiolgenomics.00041.2007.

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Genre: Journal Article
Alternative Title : Physiol Genomics

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 Creators:
Adjaye, James1, Author           
Herwig, Ralf2, Author           
Brink, Thore C.3, Author           
Herrmann, Doris, Author
Greber, Boris4, Author
Sudheer, Smita5, Author           
Groth, Detlef4, Author
Carnwath, Joseph W., Author
Lehrach, Hans3, Author           
Niermann, Heiner, Author
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              
2Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
4Max Planck Society, ou_persistent13              
5Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

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Free keywords: Oocytes; Microarrays; Cross-species hybridization
 Abstract: The present study investigated mRNA expression profiles of bovine oocytes and blastocysts by using a cross-species hybridization approach employing an array consisting of 15,529 human cDNAs as probe, thus enabling the identification of conserved genes during human and bovine preimplantation development. Our analysis revealed 419 genes that were expressed in both oocytes and blastocysts. The expression of 1,324 genes was detected exclusively in the blastocyst, in contrast to 164 in the oocyte including a significant number of novel genes. Genes indicative for transcriptional and translational control (ELAVL4, TACC3) were overexpressed in the oocyte, whereas cellular trafficking (SLC2A14, SLC1A3), proteasome (PSMA1, PSMB3), cell cycle (BUB3, CCNE1, GSPT1), and protein modification and turnover (TNK1, UBE3A) genes were found to be overexpressed in blastocysts. Transcripts implicated in chromatin remodeling were found in both oocytes (NASP, SMARCA2) and blastocysts (H2AFY, HDAC7A). The trophectodermal markers PSG2 and KRT18 were enriched 5- and 50-fold in the blastocyst. Pathway analysis revealed differential expression of genes involved in 107 distinct signaling and metabolic pathways. For example, phosphatidylinositol signaling and gluconeogenesis were prominent pathways identified in the blastocyst. Expression patterns in bovine and human blastocysts were to a large extent identical. This analysis compared the transcriptomes of bovine oocytes and blastocysts and provides a solid foundation for future studies on the first major differentiation events in blastocysts and identification of a set of markers indicative for regular mammalian development.

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Language(s): eng - English
 Dates: 2007-10-22
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
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Title: Physiological Genomics
  Alternative Title : Physiol Genomics
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 31 (2) Sequence Number: - Start / End Page: 315 - 327 Identifier: ISSN: 1094-8341