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  A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks

Poustka, A. J., Kühn, A., Groth, D., Weise, V., Yaguchi, S., Burke, R. D., et al. (2007). A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks. Genome Biology, 8(5), R85-R85. doi:10.1186/gb-2007-8-5-r85.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-81CA-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-81CB-C
Genre: Journal Article
Alternative Title : Genome Biol

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 Creators:
Poustka, Albert J.1, Author              
Kühn, Alexander2, Author              
Groth, Detlef3, Author
Weise, Vesna3, Author
Yaguchi, Shunsuke, Author
Burke, Robert D., Author
Herwig, Ralf4, Author              
Lehrach, Hans5, Author              
Panopoulou, Georgia1, Author              
Affiliations:
1Evolution and Development (Albert Poustka), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479650              
2Systems Biology (Christoph Wierling), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479656              
3Max Planck Society, ou_persistent13              
4Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              
5Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Development; Genome studies
 Abstract: Background The genome of the sea urchin Strongylocentrotus purpuratus has recently been sequenced because it is a major model system for the study of gene regulatory networks. Embryonic expression patterns for most genes are unknown, however. Results Using large-scale screens on arrays carrying 50% to 70% of all genes, we identified novel territory-specific markers. Our strategy was based on computational selection of genes that are differentially expressed in lithium-treated embryos, which form excess endomesoderm, and in zinc-treated embryos, in which endomesoderm specification is blocked. Whole-mount in situ hybridization (WISH) analysis of 700 genes indicates that the apical organ region is eliminated in lithium-treated embryos. Conversely, apical and specifically neural markers are expressed more broadly in zinc-treated embryos, whereas endomesoderm signaling is severely reduced. Strikingly, the number of serotonergic neurons is amplified by at least tenfold in zinc-treated embryos. WISH analysis further indicates that there is crosstalk between the Wnt (wingless int), Notch, and fibroblast growth factor signaling pathways in secondary mesoderm cell specification and differentiation, similar to signaling cascades that function during development of presomitic mesoderm in mouse embryogenesis. We provide differential expression data for more than 4,000 genes and WISH patterns of more than 250 genes, and more than 2,400 annotated WISH images. Conclusion Our work provides tissue-specific expression patterns for a large fraction of the sea urchin genes that have not yet been included in existing regulatory networks and await functional integration. Furthermore, we noted neuron-inducing activity of zinc on embryonic development; this is the first observation of such activity in any organism.

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Language(s): eng - English
 Dates: 2007-05-16
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
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Title: Genome Biology
  Alternative Title : Genome Biol
Source Genre: Journal
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Pages: - Volume / Issue: 8 (5) Sequence Number: - Start / End Page: R85 - R85 Identifier: ISSN: 1465-6906