English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Detection of novel skeletogenesis target genes by comprehensive analysis of a Runx2−/− mouse model

Hecht, J., Seitz, V., Urban, M., Wagner, F., Robinson, P. N., Stiege, A., et al. (2007). Detection of novel skeletogenesis target genes by comprehensive analysis of a Runx2−/− mouse model. Gene Expression Patterns: A Section of Brain Research Devoted to Patterns of Expression of Genes during the Development, Maturity and Aging of the Central Nervous System, 7(1 - 2), 102-112. doi:doi:10.1016/j.modgep.2006.05.014.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8286-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8287-B
Genre: Journal Article
Alternative Title : Gene Expr. Patterns

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hecht, Jochen1, Author              
Seitz, Volkhard1, Author              
Urban, Maren2, Author
Wagner, F., Author
Robinson, P. N.1, Author              
Stiege, A.1, Author              
Dieterich, C.2, Author
Kornak, Uwe1, Author              
Wilkening, Ulrich2, Author
Brieske, Norbert1, Author              
Zwingman, C.2, Author
Kidess, A.2, Author
Stricker, Sigmar1, Author              
Mundlos, Stefan1, Author              
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              
2Max Planck Society, ou_persistent13              

Content

show
hide
Free keywords: Runx2; Development; Bone; Cartilage; Osteoclasts; Gene Ontology; Skeletogenesis; Gene expression; Microarray; Quantitative RT-PCR
 Abstract: Runx2 is an essential factor for skeletogenesis and heterozygous loss causes cleidocranial dysplasia in humans and a corresponding phenotype in the mouse. Homozygous Runx2-deficient mice lack hypertrophic cartilage and bone. We compared the expression profiles of E14.5 wildtype and Runx2−/− murine embryonal humeri to identify new transcripts potentially involved in cartilage and bone development. Seventy-one differentially expressed genes were identified by two independent oligonucleotide-microarray hybridizations and quantitative RT-PCR experiments. Gene Ontology analysis demonstrated an enrichment of the differentially regulated genes in annotations to terms such as extracellular, skeletal development, and ossification. In situ hybridization on E15.5 limb sections was performed for all 71 differentially regulated genes. For 54 genes conclusive in situ hybridization results were obtained and all of them showed skeletal expression. Co-expression with Runx2 was demonstrated for 44 genes. While 41 of the 71 differentially expressed genes have a known role in bone and cartilage, we identified 21 known genes that have not yet been implicated in skeletal development and 9 entirely new transcripts. Expression in the developing skeleton was demonstrated for 21 of these genes.

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Gene Expression Patterns : A Section of Brain Research Devoted to Patterns of Expression of Genes during the Development, Maturity and Aging of the Central Nervous System
  Alternative Title : Gene Expr. Patterns
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 (1 - 2) Sequence Number: - Start / End Page: 102 - 112 Identifier: ISSN: 1567-133X