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  Oligonucleotide fingerprinting of arrayed genomic DNA sequences using LNA-modified hybridization probes

Liu, J.-P., Drungowski, M., Nyarsik, L., Schwartz, R., Lehrach, H., Herwig, R., et al. (2007). Oligonucleotide fingerprinting of arrayed genomic DNA sequences using LNA-modified hybridization probes. Combinatorial Chemistry & High Throughput Screening, 10(4), 269-276. doi:10.2174/138620707780636637.

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 Creators:
Liu, Jian-Ping1, Author
Drungowski, Mario1, Author
Nyarsik, Lajos1, Author
Schwartz, Regine1, Author
Lehrach, Hans2, Author              
Herwig, Ralf3, Author              
Janitz, Michal2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
3Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              

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Free keywords: LNA; hybridization; microarray; oligonucleotide fingerprinting
 Abstract: Recently, we established a robust method for the detection of hybridization events using a DNA microarray deposited on a nanoporous membrane. Here, in a follow-up study, we demonstrate the performance of this approach on a larger set of LNA-modified oligoprobes and genomic DNA sequences. Twenty-six different LNA-modified 7-mer oligoprobes were hybridized to a set of 66 randomly selected human genomic DNA clones spotted on a nanoporous membrane slide. Subsequently, assay sensitivity analysis was performed using receiver operating characteristic (ROC) curves. Comparison of LNA-modified heptamers and DNA heptamers revealed that the LNA modification clearly improved sensitivity and specificity of hybridization experiment. Clustering analysis was applied in order to test practical performance of hybridization experiments with LNA-modified oligoprobes in recognizing similarity of genomic DNA sequences. Comparing the results with the theoretical sequence clusters, we conclude that the application of LNA-modified oligoprobes allows for reliable clustering of DNA sequences which reflects the underlying sequence homology. Our results show that LNA-modified oligoprobes can be used effectively to unravel sequence similarity of DNA sequences and thus, to characterize the content of unknown DNA libraries.

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Language(s): eng - English
 Dates: 2007
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 334908
DOI: 10.2174/138620707780636637
 Degree: -

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Title: Combinatorial Chemistry & High Throughput Screening
Source Genre: Journal
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Pages: - Volume / Issue: 10 (4) Sequence Number: - Start / End Page: 269 - 276 Identifier: ISSN: 1386-2073