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  Structure of the ribosome-bound cricket paralysis virus IRES RNA

Schüler, M., Connell, S. R., Lescoute, A., Giesebrecht, J., Dabrowski, M., Schroeer, B., et al. (2006). Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nature Structural & Molecular Biology, 13(12), 1092-1096. doi:10.1038/nsmb1177.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8321-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8322-5
Genre: Journal Article
Alternative Title : Nat Struct Mol Biol

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 Creators:
Schüler, Martin, Author
Connell, Sean R.1, Author              
Lescoute, Aurelie, Author
Giesebrecht, Jan, Author
Dabrowski, Marylena, Author
Schroeer, Birgit, Author
Mielke, Thorsten2, Author              
Penczek, Pawel A., Author
Westhof, Eric, Author
Spahn, Christian M. T.1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Imaging/Electron Microscopy (Head: Rudi Lurz/Thorsten Mielke), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              

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 Abstract: Internal ribosome entry sites (IRESs) facilitate an alternative, end-independent pathway of translation initiation. A particular family of dicistroviral IRESs can assemble elongation-competent 80S ribosomal complexes in the absence of canonical initiation factors and initiator transfer RNA. We present here a cryo-EM reconstruction of a dicistroviral IRES bound to the 80S ribosome. The resolution of the cryo-EM reconstruction, in the subnanometer range, allowed the molecular structure of the complete IRES in its active, ribosome-bound state to be solved. The structure, harboring three pseudoknot-containing domains, each with a specific functional role, shows how defined elements of the IRES emerge from a compactly folded core and interact with the key ribosomal components that form the A, P and E sites, where tRNAs normally bind. Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation.

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Language(s): eng - English
 Dates: 2006-12
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: eDoc: 312991
DOI: 10.1038/nsmb1177
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Title: Nature Structural & Molecular Biology
  Alternative Title : Nat Struct Mol Biol
Source Genre: Journal
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Pages: - Volume / Issue: 13 (12) Sequence Number: - Start / End Page: 1092 - 1096 Identifier: ISSN: 1545-9993