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  Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages

Liebe, B., Petukhova, G., Barchi, M., Bellani, M., Braselmann, H., Nakano, T., et al. (2006). Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages. Experimental Cell Research, 312(19), 3768-3781. doi:10.1016/j.yexcr.2006.07.019.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-833C-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-833D-B
Genre: Journal Article
Alternative Title : Exp. Cell Res.

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 Creators:
Liebe, Bodo1, Author
Petukhova, G., Author
Barchi, M., Author
Bellani, M., Author
Braselmann, H., Author
Nakano, T., Author
Pandita, T. K., Author
Jasin, M., Author
Fornace, A., Author
Meistrich, M. L., Author
Baarends, W. M., Author
Schimenti, J., Author
de Lange, T., Author
Keeney, S., Author
Camerini-Otero, R. D., Author
Scherthan, Harry2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: ATM; previous termBouquetnext term; DSB; previous termMeiosis; Mousenext term; Recombination; Telomere; Spermatogenesis
 Abstract: previous termMeiosisnext term pairs and segregates homologous chromosomes and thereby forms haploid germ cells to compensate the genome doubling at fertilization. Homologue pairing in many eukaryotic species depends on formation of DNA double strand breaks (DSBs) during early prophase I when telomeres begin to cluster at the nuclear periphery (previous termbouquet stage)next term. By fluorescence in situ hybridization criteria, we observe that previous termmid-preleptotene and bouquet stagenext term frequencies are altered in previous termmale micenext term deficient for proteins required for recombination, ubiquitin conjugation and telomere length control. The generally low frequencies of previous termmid-preleptotenenext term spermatocytes were significantly increased in previous termmale micenext term lacking recombination proteins SPO11, MEI1, MLH1, KU80, ubiquitin conjugating enzyme HR6B, and in previous termmicenext term with only one copy of the telomere length regulator Terf1. The previous termbouquet stagenext term was significantly enriched in Atm−/−, Spo11−/−, Mei1m1Jcs/m1Jcs, Mlh1−/−, Terf1+/− and Hr6b−/− spermatogenesis, but not in previous termmicenext term lacking recombination proteins DMC1 and HOP2, the non-homologous end-joining DNA repair factor KU80 and the ATM downstream effector GADD45a. previous termMicenext term defective in spermiogenesis (Tnp1−/−, Gmcl1−/−, Asm−/−) showed wild-type previous termmid-preleptotene and bouquetnext term frequencies. A low frequency of previous termbouquetnext term spermatocytes in Spo11−/−Atm−/− spermatogenesis suggests that DSBs contribute to the Atm−/−-correlated previous termbouquet stagenext term exit defect. Insignificant changes of previous termbouquetnext term frequencies in previous termmicenext term with defects in early previous termstagesnext term of DSB repair (Dmc1−/−, Hop2−/−) suggest that there is an ATM-specific previous terminfluence on bouquet stagenext term duration. Altogether, it appears that several pathways previous terminfluencenext term telomere previous termdynamicsnext term in mammalian previous termmeiosis.next term

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Language(s): eng - English
 Dates: 2006-11-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 307712
DOI: 10.1016/j.yexcr.2006.07.019
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Title: Experimental Cell Research
  Alternative Title : Exp. Cell Res.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 312 (19) Sequence Number: - Start / End Page: 3768 - 3781 Identifier: ISSN: 0014-4827