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  An efficient and economic enhancer mix for PCR

Ralser, M., Querfurth, R., Warnatz, H.-J., Lehrach, H., Yaspo, M.-L., & Krobitsch, S. (2006). An efficient and economic enhancer mix for PCR. Biochemical and Biophysical Research Communications (Orlando, FL), 347(3), 747-751. doi:10.1016/j.bbrc.2006.06.151.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-83B6-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-83B7-A
Genre: Journal Article
Alternative Title : Biochem Biophys Res Commun

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 Creators:
Ralser, Markus1, Author              
Querfurth, Robert2, Author              
Warnatz, Hans-Jörg3, Author              
Lehrach, Hans1, Author              
Yaspo, Marie-Laure3, Author              
Krobitsch, Sylvia4, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
3Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              
4Neurodegenerative Disorders (Sylvia Krobitsch), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479661              

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Free keywords: Polymerase chain reaction; GC-rich sequence; Enhancer; Additive; Promoter PCR; Genomic PCR; PCR template; Taq DNA polymerase
 Abstract: Polymerase chain reaction (PCR) has become a fundamental technique in molecular biology. Nonetheless, further improvements of the existing protocols are required to broaden the applicability of PCR for routine diagnostic purposes, to enhance the specificity and the yield of PCRs as well as to reduce the costs for high-throughput applications. One known problem typically reported in PCR experiments is the poor amplification of GC-rich DNA sequences. Here we designed and tested a novel effective and low-cost PCR enhancer, a concentration-dependent combination of betaine, dithiothreitol, and dimethyl sulfoxide that broadly enhanced the quantitative and/or qualitative output of PCRs. Additionally, we showed that the performances of this enhancer mix are comparable to those of commercially available PCR additives and highly effective with different DNA polymerases. Thus, we propose the routine application of this PCR enhancer mix for low- and high-throughput experiments.

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Language(s): eng - English
 Dates: 2006-09-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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 Identifiers: eDoc: 312531
DOI: 10.1016/j.bbrc.2006.06.151
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Title: Biochemical and Biophysical Research Communications (Orlando, FL)
  Alternative Title : Biochem Biophys Res Commun
Source Genre: Journal
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Pages: - Volume / Issue: 347 (3) Sequence Number: - Start / End Page: 747 - 751 Identifier: ISSN: 0006-291X