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  LNA-modified oligodeoxynucleotide hybridization with DNA microarrays printed on nanoporous membrane slides

Liu, J.-P., Guerasimova, A., Schwartz, R., Lange, M., Lehrach, H., Nyarsik, L., et al. (2006). LNA-modified oligodeoxynucleotide hybridization with DNA microarrays printed on nanoporous membrane slides. Combinatorial Chemistry & High Throughput Screening, 9(9), 591-597. doi:10.2174/138620706778249730.

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 Creators:
Liu, Jian-Ping1, Author
Guerasimova, Anna2, Author              
Schwartz, Regine1, Author
Lange, Matthias1, Author
Lehrach, Hnas1, Author
Nyarsik, Lajos1, Author
Janitz, Michal2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: LNA; oligodeoxynucleotide; hybridization; microarray; nanoporous membrane; re-sequencing
 Abstract: We report a robust method for the detection of hybridization events using a microarray-based assay on a nanoporous membrane platform. The technique is characterized by a hybridization time of only 1 hour and uses Cy5- labeled, 7-mer oligodeoxynucleotide probes modified with locked nucleic acid (LNA) nucleotides. We show that the volume of the DNA spotted onto a nanomembrane can be reduced to ∼4 nL with detectable signal intensity. Moreover, the amount of the DNA target could be reduced to 4 fmol. The described approach could dramatically increase the throughput of techniques based on sequencing by hybridization, such as oligofingerprinting, by decreasing the total number of probes that are needed for analysis of large clone sets and reduction of the sample/reagent consumption. The method is particularly advantageous when numerous hybridization-based assays must be performed for characterization of sample sets of 100,000 or more.

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Language(s): eng - English
 Dates: 2006-09
 Publication Status: Published in print
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 Identifiers: eDoc: 309269
DOI: 10.2174/138620706778249730
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Title: Combinatorial Chemistry & High Throughput Screening
Source Genre: Journal
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Pages: - Volume / Issue: 9 (9) Sequence Number: - Start / End Page: 591 - 597 Identifier: ISSN: 1386-2073