English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  The bovine IGF2 gene is differentially methylated in oocyte and sperm DNA

Gebert, C., Wrenzycki, C., Herrmann, D., Gröger, D., Reinhardt, R., Hajkova, P., et al. (2006). The bovine IGF2 gene is differentially methylated in oocyte and sperm DNA. Genomics: Internat. Journal of Gene Mapping and Nucleotide Sequencing; Emphasizing Analyses of the Human and other Complex Genomes, 88(2), 222-229. doi:10.1016/j.ygeno.2006.03.011.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-83C4-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-83C5-A
Genre: Journal Article
Alternative Title : Genomics

Files

show Files
hide Files
:
sdarticle.pdf (Any fulltext), 590KB
 
File Permalink:
-
Name:
sdarticle.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Molecular Genetics, MBMG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: MPG
License:
-

Locators

show

Creators

show
hide
 Creators:
Gebert, Claudia, Author
Wrenzycki, Christine, Author
Herrmann, Doris, Author
Gröger, Daniela, Author
Reinhardt, Richard1, Author              
Hajkova, Petra, Author
Lucas-Hahn, Andrea, Author
Carnwath, Joseph, Author
Lehrach, Hans2, Author              
Niemann, Heiner, Author
Affiliations:
1High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Content

show
hide
Free keywords: IGF2; Differentially methylated region; Cattle
 Abstract: The insulin-like growth factor 2 gene (IGF2) encodes an essential growth factor and is imprinted in various mammalian species. Differentially methylated regions (DMRs) are often located within CpG islands and are critically involved in the regulation of monoallelic Igf2 expression in the mouse. Only partial sequence information is available for the bovine IGF2 gene and no DMR has currently been identified. The goal of this study was to identify a DMR within the bovine IGF2 gene as a prerequisite for further studies on gene-specific methylation patterns during preimplantation development. Here we describe the sequence analysis of a CpG-rich DNA fragment from the 5′ untranslated region spanning exons and introns 4 and 5 and the identification of a previously unknown DMR in exon 10 of the bovine IGF2 gene. Bisulfite analysis revealed that this DMR is differentially methylated in mature oocytes and sperm. The identification of an intragenic DMR within a developmentally important gene such as the bovine IGF2 gene provides a useful tool to evaluate the methylation patterns of embryos derived in vivo and in vitro. Our study is the first report of a differentially methylated region in a bovine imprinted gene discovered by the analysis of female and male gametes.

Details

show
hide
Language(s): eng - English
 Dates: 2006-08-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 305166
DOI: 10.1016/j.ygeno.2006.03.011
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genomics : Internat. Journal of Gene Mapping and Nucleotide Sequencing ; Emphasizing Analyses of the Human and other Complex Genomes
  Alternative Title : Genomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 88 (2) Sequence Number: - Start / End Page: 222 - 229 Identifier: ISSN: 0888-7543