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  Age-specific hormonal decline is accompanied by transcriptional changes in human sebocytes in vitro

Makrantonaki, E., Adjaye, J., Herwig, R., Brink, T. C., Groth, D., Hultschig, C., et al. (2006). Age-specific hormonal decline is accompanied by transcriptional changes in human sebocytes in vitro. Aging Cell, 5(4), 331-344. doi:10.1111/j.1474-9726.2006.00223.x.

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 Creators:
Makrantonaki, Evgenia, Author
Adjaye, James1, Author              
Herwig, Ralf2, Author              
Brink, Thore C.3, Author              
Groth, Detlef, Author
Hultschig, Claus4, Author
Lehrach, Hans3, Author              
Zouboulis, Christos C., Author
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              
2Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
4Max Planck Society, ou_persistent13              

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Free keywords: cDNA microarray; c-Myc; FN1; growth factors; hormonal aging; neurodegenerative diseases, sebaceous cells; sebaceous lipids; signaling pathways; steroids
 Abstract: The importance of hormones in endogenous aging has been displayed by recent studies performed on animal models and humans. To decipher the molecular mechanisms involved in aging we maintained human sebocytes at defined hormone-substituted conditions that corresponded to average serum levels of females from 20 (f20) to 60 (f60) years of age. The corresponding hormone receptor expression was demonstrated by reverse transcription–polymerase chain reaction (RT–PCR), Western blotting and immunocytochemistry. Cells at f60 produced significantly lower lipids than at f20. Increased mRNA and protein levels of c-Myc and increased protein levels of FN1, which have been associated with aging, were detected in SZ95 sebocytes at f60 compared to those detected at f20 after 5 days of treatment. Expression profiling employing a cDNA microarray composed of 15 529 cDNAs identified 899 genes with altered expression levels at f20 vs. f60. Confirmation of gene regulation was performed by real-time RT–PCR. The functional annotation of these genes according to the Gene Ontology identified pathways related to mitochondrial function, oxidative stress, ubiquitin-mediated proteolysis, cell cycle, immune responses, steroid biosynthesis and phospholipid degradation – all hallmarks of aging. Twenty-five genes in common with those identified in aging kidneys and several genes involved in neurodegenerative diseases were also detected. This is the first report describing the transcriptome of human sebocytes and its modification by a cocktail of hormones administered in age-specific levels and provides an in vitro model system, which approximates some of the hormone-dependent changes in gene transcription that occur during aging in humans.

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Language(s): eng - English
 Dates: 2006-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 309351
DOI: 10.1111/j.1474-9726.2006.00223.x
 Degree: -

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Title: Aging Cell
Source Genre: Journal
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Pages: - Volume / Issue: 5 (4) Sequence Number: - Start / End Page: 331 - 344 Identifier: ISSN: 1474-9718