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  Gene identification and analysis of transcripts differentially regulated in fracture healing by EST sequencing in the domestic sheep

Hecht, J., Kuhl, H., Haas, S. A.., Bauer, S., Poustka, A. J., Lienau, J., et al. (2006). Gene identification and analysis of transcripts differentially regulated in fracture healing by EST sequencing in the domestic sheep. BMC Genomics, 7, 172-192. doi:10.1186/1471-2164-7-172.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-83FF-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8400-D
Genre: Journal Article
Alternative Title : BMC genomics

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 Creators:
Hecht, Jochen1, Author              
Kuhl, Heiner2, Author              
Haas, Stefan A .3, Author
Bauer, Sebastian3, Author
Poustka, Albert J.4, Author              
Lienau, Jasmin, Author
Schell, Hanna, Author
Stiege, Asita C.1, Author              
Seitz, Volkhard1, Author              
Reinhardt, Richard5, Author              
Duda, Georg N., Author
Mundlos, Stefan1, Author              
Robinson, Peter N.1, Author              
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              
2Sequencing (Head: Bernd Timmermann), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479670              
3Max Planck Society, ou_persistent13              
4Evolution and Development (Albert Poustka), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479650              
5High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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 Abstract: Background The sheep is an important model animal for testing novel fracture treatments and other medical applications. Despite these medical uses and the well known economic and cultural importance of the sheep, relatively little research has been performed into sheep genetics, and DNA sequences are available for only a small number of sheep genes. Results In this work we have sequenced over 47 thousand expressed sequence tags (ESTs) from libraries developed from healing bone in a sheep model of fracture healing. These ESTs were clustered with the previously available 10 thousand sheep ESTs to a total of 19087 contigs with an average length of 603 nucleotides. We used the newly identified sequences to develop RT-PCR assays for 78 sheep genes and measured differential expression during the course of fracture healing between days 7 and 42 postfracture. All genes showed significant shifts at one or more time points. 23 of the genes were differentially expressed between postfracture days 7 and 10, which could reflect an important role for these genes for the initiation of osteogenesis. Conclusion The sequences we have identified in this work are a valuable resource for future studies on musculoskeletal healing and regeneration using sheep and represent an important head-start for genomic sequencing projects for Ovis aries, with partial or complete sequences being made available for over 5,800 previously unsequenced sheep genes.

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Language(s): eng - English
 Dates: 2006-07-05
 Publication Status: Published in print
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 Identifiers: eDoc: 307744
DOI: 10.1186/1471-2164-7-172
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Title: BMC Genomics
  Alternative Title : BMC genomics
Source Genre: Journal
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Pages: - Volume / Issue: 7 Sequence Number: - Start / End Page: 172 - 192 Identifier: ISSN: 1471-2164