English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins

Hu, Y.-H., Warnatz, H.-J., Vanhecke, D., Wagner, F., Fiebitz, A., Thamm, S., et al. (2006). Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins. BMC Genomics, 7, 155-155. doi:10.1186/1471-2164-7-155.

Item is

Files

show Files
hide Files
:
Hu.pdf (Any fulltext), 9MB
Name:
Hu.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: PUBLIC
License:
-

Locators

show

Creators

show
hide
 Creators:
Hu, Yu-Hui1, Author           
Warnatz, Hans-Jörg2, Author           
Vanhecke, Dominique3, Author
Wagner, Florian, Author
Fiebitz, Andrea4, Author           
Thamm, Sabine2, Author           
Kahlem, Pascal3, Author
Lehrach, Hans4, Author           
Yaspo, Marie-Laure2, Author           
Janitz, Michal4, Author           
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              
2Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              
3Max Planck Society, ou_persistent13              
4Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Content

show
hide
Free keywords: -
 Abstract: Background Trisomy of human chromosome 21 (Chr21) results in Down's syndrome, a complex developmental and neurodegenerative disease. Molecular analysis of Down's syndrome, however, poses a particular challenge, because the aneuploid region of Chr21 contains many genes of unknown function. Subcellular localization of human Chr21 proteins may contribute to further understanding of the functions and regulatory mechanisms of the genes that code for these proteins. Following this idea, we used a transfected-cell array technique to perform a rapid and cost-effective analysis of the intracellular distribution of Chr 21 proteins. Results We chose 89 genes that were distributed over the majority of 21q, ranging from RBM11 (14.5 Mb) to MCM3AP (46.6 Mb), with part of them expressed aberrantly in the Down's syndrome mouse model. Open reading frames of these genes were cloned into a mammalian expression vector with an amino-terminal His6 tag. All of the constructs were arrayed on glass slides and reverse transfected into HEK293T cells for protein expression. Co-localization detection using a set of organelle markers was carried out for each Chr21 protein. Here, we report the subcellular localization properties of 52 proteins. For 34 of these proteins, their localization is described for the first time. Furthermore, the alteration in cell morphology and growth as a result of protein over-expression for claudin-8 and claudin-14 genes has been characterized. Conclusion The cell array-based protein expression and detection approach is a cost-effective platform for large-scale functional analyses, including protein subcellular localization and cell phenotype screening. The results from this study reveal novel functional features of human Chr21 proteins, which should contribute to further understanding of the molecular pathology of Down's syndrome.

Details

show
hide
Language(s): eng - English
 Dates: 2006-06-16
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 308500
DOI: 10.1186/1471-2164-7-155
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BMC Genomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 Sequence Number: - Start / End Page: 155 - 155 Identifier: ISSN: 1471-2164