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  Liquid-based hybridization assay with real-time detection in miniaturized array platforms

Guerasimova, A., Nyarsik, L., Liu, J.-P., Schwartz, R., Lange, M., Lehrach, H., et al. (2006). Liquid-based hybridization assay with real-time detection in miniaturized array platforms. Biomolecular Engineering, 23(1), 35-40. doi:10.1016/j.bioeng.2005.09.005.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-847E-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-847F-4
Genre: Journal Article

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 Creators:
Guerasimova, Anna1, Author              
Nyarsik, Lajos2, Author
Liu, Jian-Ping2, Author
Schwartz, Regine2, Author
Lange, Matthias2, Author
Lehrach, Hnas2, Author
Janitz, Michal1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Max Planck Society, ou_persistent13              

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Free keywords: Hybridization; iFRET; Locked nucleic acids; Oligonucleotide fingerprinting
 Abstract: An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption.

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Language(s): eng - English
 Dates: 2006-03
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 271278
DOI: 10.1016/j.bioeng.2005.09.005
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Title: Biomolecular Engineering
Source Genre: Journal
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Pages: - Volume / Issue: 23 (1) Sequence Number: - Start / End Page: 35 - 40 Identifier: ISSN: 1389-0344