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  Analysis of NOD2-mediated Proteome Response to Muramyl Dipeptide in HEK293 Cells

Weichart, D., Gobom, J., Klopfleisch, S., Häsler, R., Gustavsson, N., Billmann, S., et al. (2006). Analysis of NOD2-mediated Proteome Response to Muramyl Dipeptide in HEK293 Cells. Journal of Biological Chemistry, 281(4), 2380-2389. doi:10.1074/jbc.M505986200.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-84C1-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-84C2-C
Genre: Journal Article
Alternative Title : J Biol Chem

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 Creators:
Weichart, Dieter1, Author
Gobom, Johan2, Author              
Klopfleisch, Sina, Author
Häsler, Robert, Author
Gustavsson, Niklas1, Author
Billmann, Susanne, Author
Lehrach, Hans2, Author              
Seegert, Dirk, Author
Schreiber, Stefan, Author
Rosenstiel, Philip, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Abstract: NOD2, a cytosolic receptor for the bacterial proteoglycan fragment muramyl dipeptide (MDP), plays an important role in the recognition of intracellular pathogens. Variants in the bacterial sensor domain of NOD2 are genetically associated with an increased risk for the development of Crohn disease, a human chronic inflammatory bowel disease. In the present study, global protein expression changes after MDP stimulation were analyzed by two-dimensional PAGE of total protein extracts of human cultured cells stably transfected with expression constructs encoding for wild type NOD2 (NOD2WT) or the disease-associated NOD2 L1007fsinsC (NOD2SNP13) variant. Differentially regulated proteins were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) peptide mass fingerprinting and MALDI MS/MS. The limited overlap in the responses of the NOD2-overexpressing cell lines to MDP included a down-regulation of heat shock 70-kDa protein 4. A complex pro-inflammatory program regulated by NOD2WT that encompasses a regulation of key genes involved in protein folding, DNA repair, cellular redox homeostasis, and metabolism was observed both under normal growth conditions and after stimulation with MDP. By using the comparison of NOD2WT and disease-associated NOD2SNP13 variant, we have identified a proteomic signature pattern that may further our understanding of the influence of genetic variations in the NOD2 gene in the pathophysiology of chronic inflammatory bowel disease.

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Language(s): eng - English
 Dates: 2006-01-27
 Publication Status: Published in print
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 Identifiers: eDoc: 313022
DOI: 10.1074/jbc.M505986200
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Title: Journal of Biological Chemistry
  Alternative Title : J Biol Chem
Source Genre: Journal
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Pages: - Volume / Issue: 281 (4) Sequence Number: - Start / End Page: 2380 - 2389 Identifier: ISSN: 0021-9258