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  High-throughput subcellular protein localization using cell arrays

Hu, Y.-H., Vanhecke, D., Lehrach, H., & Janitz, M. (2005). High-throughput subcellular protein localization using cell arrays. Biochemical Society Transactions (London), 33(6), 1407-1408.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-854B-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-854C-E
Genre: Journal Article
Alternative Title : Biochem Soc Trans

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Hu et al. - Biochem Soc Trans.pdf (Any fulltext), 118KB
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Hu et al. - Biochem Soc Trans.pdf
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 Creators:
Hu, Y.-H.1, Author              
Vanhecke, D.2, Author
Lehrach, H.3, Author              
Janitz, M.3, Author              
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              
2Max Planck Society, ou_persistent13              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: cell array; functional genomics; high-throughput protein expression; immunohistological method; protein localization; reverse transfection
 Abstract: Accomplishment of the human and mouse genome projects resulted in accumulation of extensive gene sequence information. However, the information about the biological functions of the identified genes remains a bottleneck of the post-genomic era. Hence, assays providing simple functional information, such as localization of the protein within the cell, can be very helpful in the elucidation of its function. Transfected cell arrays offer a robust platform for protein localization studies. Open reading frames of unknown genes can be linked to a His6-tag or GFP (green fluorescent protein) reporter in expression vectors and subsequently transfected using the cell array. Cellular localization of the transfected proteins is detected either by specific anti-His-tag antibodies or directly by fluorescence of the GFP fusion protein and by counterstaining with organelle-specific dyes. The high throughput of the method in terms of information provided for every single experiment makes this approach superior to classical immunohistological methods for protein localization.

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Language(s): eng - English
 Dates: 2005-12
 Publication Status: Published in print
 Pages: -
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 Identifiers: eDoc: 271597
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Title: Biochemical Society Transactions (London)
  Alternative Title : Biochem Soc Trans
Source Genre: Journal
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Pages: - Volume / Issue: 33 (6) Sequence Number: - Start / End Page: 1407 - 1408 Identifier: ISSN: 0300-5127