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  Primary differentiation in the human blastocyst : comparative molecular portraits of inner cell mass and trophectoderm cells

Adjaye, J., Huntriss, J., Herwig, R., BenKahla, A., Brink, T. C., Wierling, C., et al. (2005). Primary differentiation in the human blastocyst: comparative molecular portraits of inner cell mass and trophectoderm cells. Stem Cells, 23(10), 1514-1525. doi:10.1634/stemcells.2005-0113.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8566-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8567-1
Genre: Journal Article

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 Creators:
Adjaye, James1, Author              
Huntriss, John, Author
Herwig, Ralf2, Author              
BenKahla, Alia3, Author              
Brink, Thore C.3, Author              
Wierling, Christoph4, Author              
Hultschig, Claus5, Author
Groth, Detlef5, Author
Yaspo, Marie-Laure6, Author              
Picton, Helen M., Author
Gosden, Roger G., Author
Lehrach, Hans3, Author              
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              
2Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
4Systems Biology (Christoph Wierling), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479656              
5Max Planck Society, ou_persistent13              
6Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              

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Free keywords: Preimplantation development; Blastocyst; Inner cell mass; Trophectoderm; Pluripotency; Embryonic stem cells; Trophoblastic stem cells; OCT4; CDX2; Differentiation; Microarrays
 Abstract: The primary differentiation event during mammalian development occurs at the blastocyst stage and leads to the delineation of the inner cell mass (ICM) and the trophectoderm (TE). We provide the first global mRNA expression data from immunosurgically dissected ICM cells, TE cells, and intact human blastocysts. Using a cDNA microarray composed of 15,529 cDNAs from known and novel genes, we identify marker transcripts specific to the ICM (e.g., OCT4/POU5F1, NANOG, HMGB1, and DPPA5) and TE (e.g., CDX2, ATP1B3, SFN, and IPL), in addition to novel ICM- and TE-specific expressed sequence tags. The expression patterns suggest that the emergence of pluripotent ICM and TE cell lineages from the morula is controlled by metabolic and signaling pathways, which include inter alia, WNT, mitogen-activated protein kinase, transforming growth factor-beta, NOTCH, integrin-mediated cell adhesion, phosphatidylinositol 3-kinase, and apoptosis. These data enhance our understanding of the first step in human cellular differentiation and, hence, the derivation of both embryonic stem cells and trophoblastic stem cells from these lineages.

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Language(s): eng - English
 Dates: 2005-11-10
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 268494
DOI: 10.1634/stemcells.2005-0113
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Title: Stem Cells
Source Genre: Journal
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Pages: - Volume / Issue: 23 (10) Sequence Number: - Start / End Page: 1514 - 1525 Identifier: ISSN: 1066-5099