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  Introduction of DNA into Actinomycetes by bacterial conjugation from E. coli—An evaluation of various transfer systems

Blaesing, F., Mühlenweg, A., Vierling, S., Ziegelin, G., Pelzer, S., & Lanka, E. (2005). Introduction of DNA into Actinomycetes by bacterial conjugation from E. coli—An evaluation of various transfer systems. Journal of Biotechnology, 120(2), 146-161. doi:10.1016/j.jbiotec.2005.06.023.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8569-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-856A-C
Genre: Journal Article
Alternative Title : J Biotech

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Blaesing et al. - J Biotechnol.pdf (Any fulltext), 383KB
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 Creators:
Blaesing, Franca1, Author              
Mühlenweg, Agnes, Author
Vierling, Silke, Author
Ziegelin, Günter2, Author
Pelzer, Stefan, Author
Lanka, Erich2, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Max Planck Society, ou_persistent13              

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Free keywords: Novel conjugative binary vector systems; Intergeneric transfer; Streptomyces/Actinomycetales
 Abstract: Gene transfer is a basic requirement for optimizing bioactive natural substances produced by an increasing number of industrially used microorganisms. We have analyzed quantitatively horizontal gene transfer from Escherichia coli to Actinomycetes. The efficiencies of DNA transfer of four different systems were compared that consist of conjugative and mobilizable plasmids with a broad-host range. Three novel binary vector set-ups were constructed based on: (i) the IncQ group of mobilizable plasmids (RSF1010), (ii) IncQ-like pTF-FC2 and (iii) pSB102 that belongs to a new class of broad-host-range plasmids. The established system based on the IncPα group of conjugative plasmids served as the reference. For all plasmids constructed, we confirmed the functional integrity of the selected transfer machineries by intrageneric matings between E. coli strains. We demonstrate that the transfer systems introduced in this study are efficient in mediating gene transfer from E. coli to Actinomycetes and are possible alternatives for gene transfer into Actinomycetes for which the IncPα-based transfer system is not applicable. The use of plasmids that integrate into the recipients’ chromosomes compared to that of plasmids replicating autonomously is shown to allow the access to a wider range of hosts.

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Language(s): eng - English
 Dates: 2005-11-04
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 268502
DOI: 10.1016/j.jbiotec.2005.06.023
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Title: Journal of Biotechnology
  Alternative Title : J Biotech
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 120 (2) Sequence Number: - Start / End Page: 146 - 161 Identifier: ISSN: 0168-1656