English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  First genome data from uncultured upland soil cluster alpha methanotrophs provide further evidence for a close phylogenetic relationship to Methylocapsa acidiphila B2 and for high-affinity methanotrophy involving particulate methane monooxygena

Ricke, P., Kube, M., Nakagawa, S., Erkel, C., Reinhardt, R., & Liesack, W. (2005). First genome data from uncultured upland soil cluster alpha methanotrophs provide further evidence for a close phylogenetic relationship to Methylocapsa acidiphila B2 and for high-affinity methanotrophy involving particulate methane monooxygena. Applied and Environmental Microbiology, 71(11), 7472-7482. doi:10.1128/AEM.71.11.7472-7482.2005.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8571-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8572-7
Genre: Journal Article
Alternative Title : Appl Environ Microbiol

Files

show Files
hide Files
:
7472.pdf (Any fulltext), 775KB
 
File Permalink:
-
Name:
7472.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Molecular Genetics, Berlin; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: INSTITUT
License:
-

Locators

show

Creators

show
hide
 Creators:
Ricke, Peter1, Author
Kube, Michael2, Author              
Nakagawa, Satoshi, Author
Erkel, Christoph1, Author
Reinhardt, Richard2, Author              
Liesack, Werner1, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

Content

show
hide
Free keywords: -
 Abstract: Members of upland soil cluster alpha (USC alpha) are assumed to be methanotrophic bacteria (MB) adapted to the trace level of atmospheric methane. So far, these MB have eluded all cultivation attempts. While the 16S rRNA phylogeny of USC alpha members is still not known, phylogenies constructed for the active-site polypeptide (encoded by pmoA) of particulate methane monooxygenase (pMMO) placed USC alpha next to the alphaproteobacterial Methylocapsa acidiphila B2. To assess whether the pmoA tree reflects the evolutionary identity of USC alpha, a 42-kb genomic contig of a USC alpha representative was obtained from acidic forest soil by screening a metagenomic fosmid library of 250,000 clones using pmoA-targeted PCR. For comparison, a 101-kb genomic contig from M. acidiphila was analyzed, including the pmo operon. The following three lines of evidence confirmed a close phylogenetic relationship between USC alpha and M. acidiphila: (i) tetranucleotide frequency patterns of 5-kb genomic subfragments, (ii) annotation and comparative analysis of the genomic fragments against all completely sequenced genomes available in public domain databases, and (iii) three single gene phylogenies constructed using the deduced amino acid sequences of a putative prephenate dehydratase, a staphylococcal-like nuclease, and a putative zinc metalloprotease. A comparative analysis of the pmo operons of USC alpha and M. acidiphila corroborated previous reports that both the pmo operon structure and the predicted secondary structure of deduced pMMO are highly conserved among all MB.

Details

show
hide
Language(s): eng - English
 Dates: 2005-11-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 273069
DOI: 10.1128/AEM.71.11.7472-7482.2005
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied and Environmental Microbiology
  Alternative Title : Appl Environ Microbiol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 71 (11) Sequence Number: - Start / End Page: 7472 - 7482 Identifier: ISSN: 0099-2240