English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Unsaturated fatty acids are inhibitors of bacterial conjugation

Fernandez-Lopez, R., Machón, C., Longshaw, C. M., Martin, S., Molin, S., Zechner, E. L., et al. (2005). Unsaturated fatty acids are inhibitors of bacterial conjugation. Microbiology, 151(11), 3517-3526. doi:10.1099/mic.0.28216-0.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8574-3 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8575-1
Genre: Journal Article

Files

show Files
hide Files
:
Fernandez-Lopez et al. - Microbiology.pdf (Any fulltext), 166KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0010-8573-5
Name:
Fernandez-Lopez et al. - Microbiology.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
eDoc_access: PUBLIC
License:
-

Locators

show

Creators

show
hide
 Creators:
Fernandez-Lopez, Raul, Author
Machón, Cristina, Author
Longshaw, Christopher M., Author
Martin, Steve, Author
Molin, Soren, Author
Zechner, Ellen L., Author
Espinosa, Manuel, Author
Lanka, Erich1, Author
de la Cruz, Fernando, Author
Affiliations:
1Max Planck Society, ou_persistent13              

Content

show
hide
Free keywords: -
 Abstract: This report describes a high-throughput assay to identify substances that reduce the frequency of conjugation in Gram-negative bacteria. Bacterial conjugation is largely responsible for the spread of multiple antibiotic resistances in human pathogens. Conjugation inhibitors may provide a means to control the spread of antibiotic resistance. An automated conjugation assay was developed that used plasmid R388 and a laboratory strain of Escherichia coli as a model system, and bioluminescence as a reporter for conjugation activity. Frequencies of conjugation could be measured continuously in real time by the amount of light produced, and thus the effects of inhibitory compounds could be determined quantitatively. A control assay, run in parallel, allowed elimination of compounds affecting cell growth, plasmid stability or gene expression. The automated conjugation assay was used to screen a database of more than 12 000 microbial extracts known to contain a wide variety of bioactive compounds (the NatChem library). The initial hit rate was 1·4 %. From these, 48 extracts containing active compounds and representing a variety of organisms and extraction conditions were subjected to fractionation (24 fractions per extract). The 52 most active fractions were subjected to a secondary analysis to determine the range of plasmid inhibition. Plasmids R388, R1 and RP4 were used as representatives of a variety of plasmid transfer systems. Only one fraction (of complex composition) affected transfer of all three plasmids, while four other fractions were active against two of them. Two separate compounds were identified from these fractions: linoleic acid and dehydrocrepenynic acid. Downstream analysis showed that the chemical class of unsaturated fatty acids act as true inhibitors of conjugation.

Details

show
hide
Language(s): eng - English
 Dates: 2005-11
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 271177
DOI: 10.1099/mic.0.28216-0
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Microbiology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 151 (11) Sequence Number: - Start / End Page: 3517 - 3526 Identifier: ISSN: 1350-0872