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  Method for qualitative comparisons of protein mixtures based on enzyme-catalyzed stable-isotope incorporation

Mirgorodskaya, E., Wanker, E. E., Otto, A., Lehrach, H., & Gobom, J. (2005). Method for qualitative comparisons of protein mixtures based on enzyme-catalyzed stable-isotope incorporation. Journal of Proteome Research, 4(6), 2109-2116. doi:10.1021/pr050219i S1535-3893(05)00219-8.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8581-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8582-3
Genre: Journal Article
Alternative Title : J Proteome Res

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Mirgorodskaya et al. - J Proteome Res.pdf (Any fulltext), 251KB
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Mirgorodskaya et al. - J Proteome Res.pdf
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 Creators:
Mirgorodskaya, Ekaterina1, Author
Wanker, Erich E., Author
Otto, Albrecht, Author
Lehrach, Hans2, Author              
Gobom, Johan2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: O labeling; stable isotope; protein interactions; comparative proteomics; mass spectrometry; MALDI; nano-liquid chromatography
 Abstract: Determining which proteins are unique among one or several protein populations is an often-encountered task in proteomics. To this purpose, we present a new method based on trypsin-catalyzed incorporation of the stabile isotope 18O in the C-termini of tryptic peptides, followed by LC-MALDI MS analysis. The analytical strategy was designed such that proteins unique to a given population out of several can be assigned in a single experiment by the isotopic signal intensity distributions of their tryptic peptides in the recorded mass spectra. The method is demonstrated for protein-protein interaction analysis, in which the differential isotope labeling was used to distinguish endogenous human brain proteins interacting with a recombinant bait protein from nonbiospecific background binders.

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Language(s): eng - English
 Dates: 2005-10-08
 Publication Status: Published in print
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Title: Journal of Proteome Research
  Alternative Title : J Proteome Res
Source Genre: Journal
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Pages: - Volume / Issue: 4 (6) Sequence Number: - Start / End Page: 2109 - 2116 Identifier: ISSN: 1535-3893