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  A human protein-protein interaction network : a resource for annotating the proteome

Stelzl, U., Worm, U., Lalowski, M., Haenig, C., Brembeck, F. H., Goehler, H., et al. (2005). A human protein-protein interaction network: a resource for annotating the proteome. Cell, 122(6), 957-968. doi:10.1016/j.cell.2005.08.029.

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Stelzl et al. - Cell.pdf (Any fulltext), 730KB
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Stelzl, Ulrich1, Author           
Worm, Uwe, Author
Lalowski, Maciej, Author
Haenig, Christian, Author
Brembeck, Felix H., Author
Goehler, Heike, Author
Stroedicke, Martin, Author
Zenkner, Martina, Author
Schoenherr, Anke, Author
Koeppen, Susanne2, Author
Timm, Jan, Author
Mintzlaff, Sascha, Author
Abraham, Claudia, Author
Bock, Nicole, Author
Kietzmann, Silvia2, Author
Goedde, Astrid, Author
Toksöz, Engin, Author
Droege, Anja3, Author           
Krobitsch, Sylvia4, Author           
Korn, Bernhard, Author
Birchmeier, Walter, AuthorLehrach, Hans3, Author           Wanker, Erich E.2, Author more..
Affiliations:
1Molecular Interaction Networks (Ulrich Stelzl), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479660              
2Max Planck Society, ou_persistent13              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
4Neurodegenerative Disorders (Sylvia Krobitsch), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479661              

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 Abstract: Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and coimmunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes.

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Language(s): eng - English
 Dates: 2005-09-15
 Publication Status: Published in print
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 Identifiers: eDoc: 275687
DOI: 10.1016/j.cell.2005.08.029
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Title: Cell
Source Genre: Journal
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Pages: - Volume / Issue: 122 (6) Sequence Number: - Start / End Page: 957 - 968 Identifier: ISSN: 0092-8674