English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  : Expression and functional analysis of genes deregulated in mouse placental overgrowth models: Car2 and Ncam1

Singh, U., Sun, T., Shi, W., Schulz, R., Nuber, U. A., Varanou, A., et al. (2005).: Expression and functional analysis of genes deregulated in mouse placental overgrowth models: Car2 and Ncam1. Developmental Dynamics, 234(4), 1034-1045. doi:10.1002/dvdy.20597.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : Dev Dyn

Files

show Files
hide Files
:
fulltext_ID=112133841&PLACEBO=IE.pdf (Any fulltext), 767KB
 
File Permalink:
-
Name:
fulltext_ID=112133841&PLACEBO=IE.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Molecular Genetics, MBMG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: MPG
License:
-

Locators

show

Creators

show
hide
 Creators:
Singh, Umashankar, Author
Sun, Tong, Author
Shi, Wei, Author
Schulz, Ralph1, Author
Nuber, Ulrike A.2, Author              
Varanou, Aikaterini, Author
Hemberger, Myriam C., Author
Elliott, Rosemary W., Author
Ohta, Hiroshi, Author
Wakayama, Teruhiko, Author
Fundele, Reinald2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

Content

show
hide
Free keywords: Car2 Ncam1 placental growth functional redundancy
 Abstract: Different causes, such as maternal diabetes, cloning by nuclear transfer, interspecific hybridization, and deletion of some genes such as Esx1, Ipl, or Cdkn1c, may underlie placental overgrowth. In a previous study, we carried out comparative gene expression analysis in three models of placental hyperplasias, cloning, interspecies hybridization (IHPD), and Esx1 deletion. This study identified a large number of genes that exhibited differential expression between normal and enlarged placentas; however, it remained unclear how altered expression of any specific gene was related to any specific placental phenotype. In the present study, we focused on two genes, Car2 and Ncam1, which both exhibited increased expression in interspecies and cloned hyperplastic placentas. Apart from a detailed expression analysis of both genes during normal murine placentation, we also assessed morphology of placentas that were null for Car2 or Ncam1. Finally, we attempted to rescue placental hyperplasia in a congenic model of IHPD by decreasing transcript levels of Car2 or Ncam1. In situ analysis showed that both genes are expressed mainly in the spongiotrophoblast, however, expression patterns exhibited significant variability during development. Contrary to expectations, homozygous deletion of either Car2 or Ncam1 did not result in placental phenotypes. However, expression analysis of Car3 and Ncam2, which can take over the function of Car2 and Ncam1, respectively, indicated a possible rescue mechanism, as Car3 and Ncam2 were expressed in spongiotrophoblast of Car2 and Ncam1 mutant placentas. On the other hand, downregulation of either Car2 or Ncam1 did not rescue any of the placental phenotypes of AT24 placentas, a congenic model for interspecies hybrid placentas. This strongly suggested that altered expression of Car2 and Ncam1 is a downstream event in placental hyperplasia.

Details

show
hide
Language(s): eng - English
 Dates: 2005-08-26
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 271598
DOI: 10.1002/dvdy.20597
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Developmental Dynamics
  Alternative Title : Dev Dyn
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 234 (4) Sequence Number: - Start / End Page: 1034 - 1045 Identifier: ISSN: 1058-8388